Identification of a coactivator that increases activation of transcription by serum response factor and GAL4-VP16 in vitro.
Identification of a coactivator that increases activation of transcription by serum response factor and GAL4-VP16 in vitro.
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体外鉴定可增加血清反应因子和 GAL4-VP16 转录激活的共激活剂。
DOI：
10.1073/pnas.89.12.5291
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发表时间：
1992
影响因子：
11.1
通讯作者：
Pyrwes,R
中科院分区：
文献类型：
--
作者：
Zhu,H;Pyrwes,R
我们已经确定了HeLa细胞核提取物中的活性，其通过血清反应因子（SRF）增加体外转录的激活。这种共激活因子活性，称为CoS，不影响基础（TATA只）转录。CoS最初与转录因子TFIIE和-F一起分级分离，但进一步纯化以将其与TFIIE和-F以及任何其他一般转录因子活性分离。我们发现CoS对SRF激活不是特异性的，因为它也增加了嵌合GAL 4-VP 16激活剂的转录激活。CoS和最近确定的coactivators之间的差异进行了讨论。
We have identified an activity in HeLa cell nuclear extracts that increases activation of transcription in vitro by serum response factor (SRF). This coactivator activity, termed CoS, did not affect basal (TATA only) transcription. CoS initially fractionated with transcription factors TFIIE and -F but was further purified to separate it from both TFIIE and -F as well as any other general transcription factor activity. We found that CoS was not specific for SRF activation, since it also increased transcriptional activation by the chimeric GAL4-VP16 activator. Differences among CoS and recently identified coactivators are discussed.