Taxonomy of mycelial actinobacteria isolated from Saharan soils and their efficiency to reduce aflatoxin B1 content in a solid-based medium
Taxonomy of mycelial actinobacteria isolated from Saharan soils and their efficiency to reduce aflatoxin B1 content in a solid-based medium
复制标题
DOI：
10.1007/s13213-017-1253-7
复制
发表时间：
2017-03-01
影响因子：
3
通讯作者：
Mathieu, Florence
中科院分区：
文献类型：
--
作者：
Lahoum, Abdelhadi;Verheecke-Vaessen, Carol;Mathieu, Florence
黄曲霉毒素 B1 (AFB1) 是一种由丝状真菌产生的致癌化合物。为了减少食品中AFB1的出现，对13株菌丝放线菌进行了体外降低AFB1含量的功效测试；所有这些都是从阿尔及利亚的撒哈拉土壤中分离出来的。首先，基于16S rRNA基因的形态学研究和分子分析表明，这些菌株属于Actinomadura属、Nocardiopsis属、Nonomuraea属、Saccharothrix属和Streptomyces属。其次，在 ISP2 培养基中研究了每种菌株降低纯 AFB1 含量的功效。在添加 AFB1 的培养基（5 ppm AFB1）上于 30°C 孵育 4 天后，提取 AFB1 并进行定量。所有菌株的 AFB1 含量均降低 (42.9-97.6%)。三种最有效的还原剂（94.9-97.6%）是属于链霉菌属的两种菌株和一种属于糖丝菌属的菌株。其中，菌株 ACD6 和 ABH19 没有表现出吸附机制，表明存在潜在的降解机制。这些发现使我们建议这些放线菌菌株可用作减少 AFB1 含量的净化处理。
Aflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce AFB1 occurrence in foodstuffs, 13 strains of mycelial actinobacteria were tested in vitro for the efficacy to reduce AFB1 content; all were isolated from the Saharan soils of Algeria. Firstly, morphological study and molecular analysis, based on the 16S rRNA gene, indicated that these strains belong to Actinomadura, Nocardiopsis, Nonomuraea, Saccharothrix and Streptomyces genera. Secondly, each strain's efficacy to reduce pure AFB1 content was studied in ISP2-medium. After a 4-day incubation at 30A degrees C on AFB1-supplemented medium (5 ppm of AFB1), AFB1 was extracted and quantified. AFB1 content was reduced by all strains (42.9-97.6%). The three most efficient reducers (94.9-97.6%) were two strains belonging to the genus Streptomyces and one to the genus Saccharothrix. Among the latter, strains ACD6 and ABH19 showed no adsorption mechanism involved, suggesting a potential degradation mechanism. These findings led us to suggest that these actinobacterial strains could be used as decontamination treatments for the reduction of AFB1 content.