The influence of masticatory hypofunction on developing rat craniofacial structure
The influence of masticatory hypofunction on developing rat craniofacial structure
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DOI：
10.1016/j.ijom.2010.02.011
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发表时间：
2010-06-01
影响因子：
2.4
通讯作者：
Chiu, W. C.
中科院分区：
文献类型：
--
作者：
Tsai, C. Y.;Yang, L. Y.;Chiu, W. C.
本研究应用A型肉毒神经毒素(BoNT/A)选择性地评价局限性咀嚼萎缩和轻瘫对颅面生长发育的影响。60只4周龄、体重约120g的生长期大鼠按随机数字表法分为：(Long-Evans，N=15只/组)I(Mb+TNS)组、II(MNS+Tb组)、II(MNS+Tb组)、I(Mb+TNS组)、I(Mb+TNS组)、II(MNS+Tb组)。III(Mb+Tb)，IV(MNS+Tns)，其中Mb或Tb为BONT/A注射的咬肌或颞肌(1.0U/肌，2.5ml)，MNS或TNS为注射生理盐水的肌肉(2.5ml)。7周后处死成年大鼠，解剖肌肉，测量颅骨、上颌骨和下颌骨的人体测量值，生长期动物体重变化无统计学意义，注射BoNT/A的Mb和Tb肌肉的平均咀嚼肌重较小。由咬肌和颞肌植入的骨结构的人体测量显示了显著的治疗效果。测量显示出典型的面部形态特征，即上面部较短，下面部较长，下颌长度和升支高度延长，双冠和双尖宽度缩小。结果表明，BONT/A导致局限性咀嚼肌萎缩改变了颅面的生长和发育。
The purpose of this study was to use botulinum neurotoxin type A (BoNT/A) selectively to evaluate the influence of localized masticatory atrophy and paresis on craniofacial growth and development 60 growing rats, 4 weeks old, weighing approximately 120 g, were randomly divided according as follows (Long-Evans, N = 15 per group) I (Mb + Tns); II (Mns + Tb); III (Mb + Tb), IV (Mns + Tns), where Mb or Tb is the BoNT/A-injected masseter or temporalis muscles (1 0 U/ muscle, 2 5 ml) and Mns or Tns is the saline-injected muscles (2.5 ml). After 7 weeks, the mature rats were killed, the muscles dissected and mean muscle mass recorded Anthropometric cranial, maxillary and mandibular measurements were taken from the dried skulls Changes in animal weight during the growth period were not statistically significant The mean masticatory muscle mass was smaller for the BoNT/A-injected muscles of Mb and Tb. Anthropometric measurements of bony structures inserted by masseter and temporalis muscles revealed a significant treatment effect The measurements showed a facial morphology typical of a dolichofacial profile short upper face accompanied by a long lower face with an extended mandibular length and ramus height and constricted bicoronoidal and bigonial widths The results suggest that induction of localized masticatory muscle atrophy with BoNT/A alters craniofacial growth and development.