STABILITY OF NA+-K+-ATPASE ALPHA-SUBUNIT ISOFORMS IN EVOLUTION
STABILITY OF NA+-K+-ATPASE ALPHA-SUBUNIT ISOFORMS IN EVOLUTION
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DOI：
10.1152/ajpcell.1990.259.4.c619
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发表时间：
1990-10-01
影响因子：
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通讯作者：
FAMBROUGH, DM
中科院分区：
文献类型：
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作者：
TAKEYASU, K;LEMAS, V;FAMBROUGH, DM
编码α-半乳糖苷酶的α 2-和α 3-同种型的DNA克隆了鸡Na ~+-K ~+-ATP酶的一个亚基，并报道了其核苷酸序列和推导的氨基酸序列。这些数据与大鼠α-的可比数据之间的比较亚基亚型使得评估α-成为可能亚基异构体多样性。在α-间存在大约两倍的氨基酸序列差异。同种型在单个物种内存在差异，因为相应的α-鸟类和哺乳动物的同种型。这些数据与三倍的α-亚基基因和基本上不同的α-亚基同种型在2亿多年前鸟类和哺乳动物谱系分离之前，然后通过随后的进化保留大部分这些结构差异。这种保守的异构体特异性的结构特征的影响进行了讨论的运输功能和生物调节的Na+-K+-ATP酶。
Encoding DNA for .alpha.2- and .alpha.3-isoforms of the .alpha.-subunit of the chicken Na+-K+-ATPase have been cloned and their nucleotide sequences and deduced amino acid sequences are reported. Comparisons between these data and comparable data for the rat .alpha.-subunit isoforms make possible an assessment of .alpha.-subunit isoform diversity among vertebrates. There is approximately twice as much amino acid sequence difference between .alpha.-isoforms within a single species as there is difference between corresponding .alpha.-isoforms of bird and mammal. These data are consistent with triplication of the .alpha.-subunit gene and evolution of substantially different .alpha.-subunit isoforms before the separation of avian and mammalian lineages over 200 million years ago and then retention of the majority of these structural differences through subsequent evolution. The implications of this conservation of isoform-specific structural features are discussed in terms of transport functions and bioregulation of the Na+-K+-ATPase.