Dissection of pilus tip assembly by the FimD usher monomer.
Dissection of pilus tip assembly by the FimD usher monomer.
复制标题
DOI：
10.1016/j.jmb.2012.12.024
复制
发表时间：
2013-03-11
影响因子：
5.6
通讯作者：
Waksman, Gabriel
中科院分区：
文献类型：
--
作者：
Allen, William J.;Phan, Gilles;Hultgren, Scott J.;Waksman, Gabriel
I型菌毛是一类细菌表面结构的代表，由保守的伴侣/usher途径组装而成，在感染过程中被致尿路病原性大肠杆菌用来附着在膀胱细胞上。外膜组装平台-引座器-对于菌毛的形成至关重要，它催化菌毛亚单位的聚合，并使新生菌毛得以分泌。尽管引座者的结构特征广泛，但关于其机制仍存在一些问题，特别是其寡聚化状态，以及它如何协调毛状亚单位的有序组装。我们在这里证明了FimD引导者能够以其单体形式有效地催化体外菌毛组装。此外，通过建立不同菌毛亚基之间由引座器催化的反应动力学，我们建立了一个完整的尖端原纤维组装动力学模型，能够解释天然1型菌毛亚基的顺序。对►组装毛尖的机理进行了研究。►是FIMD的引导者，可以催化单体形式的给体链交换。►提出了一种新的供体链交换动力学模型。►获得了菌毛生物发生的完整动力学特征。
Type 1 pili are representative of a class of bacterial surface structures assembled by the conserved chaperone/usher pathway and used by uropathogenic Escherichia coli to attach to bladder cells during infection. The outer membrane assembly platform—the usher—is critical for the formation of pili, catalysing the polymerisation of pilus subunits and enabling the secretion of the nascent pilus. Despite extensive structural characterisation of the usher, a number of questions about its mechanism remain, notably its oligomerisation state, and how it orchestrates the ordered assembly of pilus subunits. We demonstrate here that the FimD usher is able to catalyse in vitro pilus assembly effectively in its monomeric form. Furthermore, by establishing the kinetics of usher-catalysed reactions between various pilus subunits, we establish a complete kinetic model of tip fibrillum assembly, able to account for the order of subunits in native type 1 pili. ► The mechanism of pilus tip assembly by FimD is investigated. ► The FimD usher can catalyse donor strand exchange in its monomeric form. ► A novel model for the kinetics of donor strand exchange is proposed. ► A complete kinetic characterisation of pilus biogenesis is achieved.
登录
查看更多内容
影响因子：
5.6
作者：
Li, HL;Qian, LP;Thanassi, DG
通讯作者：
Thanassi, DG
影响因子：
5.6
作者：
Nishiyama, Mireille;Glockshuber, Rudi
通讯作者：
Glockshuber, Rudi
影响因子：
5.6
作者：
Hahn, E;Wild, P;Müller, SA
通讯作者：
Müller, SA
影响因子：
64.8
作者：
Vetsch, M;Puorger, C;Glockshuber, R
通讯作者：
Glockshuber, R
影响因子：
3.4
作者：
Schuck, P
通讯作者：
Schuck, P