Comparative Metabolomic Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Leaf Color Development of Cymbidium sinense 'Red Sun'
Comparative Metabolomic Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Leaf Color Development of Cymbidium sinense 'Red Sun'
复制标题
比较代谢组学分析揭示了大花蕙兰叶色发育的独特类黄酮生物合成调控 - 红太阳 -
DOI：
10.3390/ijms21051869
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发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Zhu, Genfa
中科院分区：
文献类型：
--
作者：
Gao, Jie;Ren, Rui;Zhu, Genfa
彩叶是墨兰的重要观赏性状。sinense），尤其是红叶，一直受到养殖者和消费者的青睐。然而，目前对红毛忍冬红叶形成机制的研究较少。中国的本研究从植物叶片中黄酮类化合物代谢产物的变化规律、相关酶活性及基因表达等方面进行了研究。对“红太阳”从红色到黄色再到绿色的变化过程进行了研究。采用UPLC-MS/MS方法共鉴定了196种黄酮类化合物，其中包括11种花色苷类化合物。在叶色变化过程中，共鉴定出42种差异显著的代谢产物，其中28种差异代谢产物的含量为零。在花青苷生物合成途径中，15种代谢产物的含量在叶色变化过程中均呈下调趋势。在15种代谢产物中，柚皮素查耳酮、天竺葵素O-乙酰己糖苷和花青素3-O-β-d-葡萄糖苷在绿色叶期含量降至零。花青苷生物合成途径中10种酶活性的变化规律与红叶由黄变绿不同，而编码这些酶的基因在叶色变化过程中表达均下调。本研究结果揭示了黄酮类化合物相关代谢产物的类型，并对代谢产物的含量、酶活性和基因表达进行了综合分析，为育种者改良小叶锦鸡儿叶色提供了新的参考依据。红太阳
The colorful leaf is an important ornamental character of Cymbidium sinense (C. sinense), especially the red leaf, which has always been attracted by breeders and consumers. However, little is documented on the formation mechanism of the red leaf of C. sinense. In this study, the changing patterns of flavonoid-related metabolites, corresponding enzyme activities and genes expression in the leaves of C. sinense ‘Red Sun’ from red to yellow and finally to green was investigated. A total of 196 flavonoid-related metabolites including 11 anthocyanins metabolites were identified using UPLC-MS/MS-based approach. In the process of leaf color change, 42 metabolites were identified as having significantly different contents and the content of 28 differential metabolites turned to zero. In anthocyanin biosynthetic pathway, content of all 15 identified metabolites showed downregulation trend in the process of leaf color change. Among the 15 metabolites, the contents of Naringenin chalcone, Pelargonidin O-acetylhexoside and Anthocyanin 3-O-beta-d-glucoside decreased to zero in the green leaf stage. The changing pattern of enzyme activity of 10 enzymes involved in the anthocyanin biosynthetic pathway showed different trends from red leaves that have turned yellow and finally green, while the expression of genes encoding these enzymes was all down-regulated in the process of leaf color change. The results of this study revealed the types of flavonoid-related metabolites and the comprehensive analysis of metabolites content, enzyme activities and genes expression providing a new reference for breeders to improve the leaf color of C. sinense ‘Red Sun’.