Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)
Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)
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DOI：
10.1016/j.rvsc.2011.03.028
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发表时间：
2012-06-01
影响因子：
2.4
通讯作者：
Patil, N. V.
中科院分区：
文献类型：
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作者：
Nagarajan, G.;Swami, Shelesh Kumar;Patil, N. V.
以双峰驼为模板，用聚合酶链式反应(PCR)扩增了双峰驼IL-2、IL-4和干扰素-γ三种细胞因子的cDNAs，并进行了序列分析。基于氨基酸序列的关系表明，双峰驼IL-2与双峰驼IL-2在核苷酸和氨基酸水平上分别有99.5%和99.3%的同源性。以IL-4为例，与双峰驼在核苷酸和氨基酸水平上的同源性分别为99.7%和99.2%。双峰驼干扰素-γ与双峰驼在核苷酸和氨基酸水平上都有100%的同源性。基于氨基酸序列的系统发育分析表明，这些细胞因子基因与其他骆驼的亲缘关系很近。(C)2011爱思唯尔有限公司。保留所有权利。
The cDNAs of three cytokines, viz., IL-2, IL-4 and IFN-gamma from Dromedary camels were amplified by PCR using Bactrian camel sequences and subsequently cloned for sequence analysis. Relationship based on amino acid sequences revealed that Dromedary camel IL-2 shared 99.5% and 99.3% identity at the nucleotide and amino acid levels with Bactrian camel IL-2. In the case of IL-4, the identity of Dromedary camel was 99.7% and 99.2% at the nucleotide and amino acid levels, respectively with that of Bactrian camel. The Dromedary camel IFN-gamma shared 100% identity both at nucleotide and amino acid levels with Bactrian camel IFN-gamma. Phylogenetic analysis based on amino acid sequences indicated the close relationship in these cytokine genes between the Dromedary camel and other camelids. (C) 2011 Elsevier Ltd. All rights reserved.