Splicing bypasses 3′ end formation signals to allow complex gene architectures
Splicing bypasses 3′ end formation signals to allow complex gene architectures
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DOI：
10.1016/j.gene.2007.08.012
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发表时间：
2007-11-15
期刊：
影响因子：
3.5
通讯作者：
Mattick, John S.
中科院分区：
文献类型：
--
作者：
Frith, Martin C.;Carninci, Piero;Mattick, John S.
在真核生物基因组中，许多基因以复杂的重叠和交错模式排列。目前尚不清楚这些基因是否或如何避免彼此RNA加工信号的干扰并保持不同的身份。这个谜题特别适用于3'端形成位点，它固有地终止转录本，因此充当相邻基因之间的边界。我们假设转录本处理机制可以通过剪接出3'端形成位点周围的内含子来绕过该位点。我们证实了这一假设的预测：转录本延伸到3‘端位点以外的可能性取决于位于成熟转录本外显子的3’端形成信号的强度，而不是剪切出转录本的内含子的信号强度。这种绕过机制包括嵌套和交错的基因结构，以及结合邻近基因外显子的融合转录本。(c) 2007 Elsevier B.V.版权所有
Many genes are arranged in complex overlapping and interlaced patterns in eukaryotic genomes. It is unclear whether or how such genes can avoid interference from each other's RNA processing signals and retain distinct identities. This puzzle applies particularly to 3' end formation sites, which inherently terminate the transcript, and thus act as boundaries between adjacent genes. We hypothesise that the transcript processing machinery can bypass 3' end formation sites by splicing out an intron surrounding the site. We confirm a prediction of this hypothesis: the likelihood of transcripts extending beyond 3' end sites depends on the strength of 3' end formation signals located in exons in the mature transcript, but not of those in introns that are spliced out of the transcript. This bypassing mechanism pen-nits nested and interleaved gene architectures, as well as fusion transcripts that combine exons from adjacent genes. (c) 2007 Elsevier B.V. All rights reserved.