Chitosan-Poly(caprolactone) Nanofibers for Skin Repair.
Chitosan-Poly(caprolactone) Nanofibers for Skin Repair.
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DOI：
10.1039/c6tb03223k
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发表时间：
2017-03-07
期刊：
影响因子：
--
通讯作者：
Zhang M
中科院分区：
文献类型：
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作者：
Levengood SL;Erickson AE;Chang FC;Zhang M
急性和慢性真皮伤口都是一个重大的临床挑战，因此开发基于生物材料的新型皮肤替代品来促进皮肤修复至关重要。由于纳米纤维与天然细胞外基质蛋白在形态和维度上的相似性，纳米纤维作为促进皮肤再生的材料而受到关注，而天然细胞外基质蛋白对于指导皮肤伤口愈合至关重要。电纺壳聚糖-聚己内酯（CPCL）纳米纤维支架结合了壳聚糖重要的固有生物学特性和 PCL 的机械完整性和稳定性，使用小鼠皮肤切除皮肤缺损模型作为皮肤组织工程支架进行了评估。对伤口大小的总体评估、随着时间的推移缺损恢复的测量以及伤口愈合的组织学评估表明，与市售的闭塞敷料 Tegaderm 相比，CPCL 纳米纤维支架提高了伤口愈合率并促进了更完全的伤口闭合。 CPCL 纳米纤维支架代表了一种皮肤修复的仿生方法，可作为立即可用的临时基质来促进伤口闭合。这些纳米纤维支架可能具有作为皮肤替代品或作为涉及与生物制剂整合的更复杂的皮肤组织工程结构的基础的巨大潜力。与商业 Tegaderm 相比，电纺壳聚糖-聚己内酯 (CPCL) 纳米纤维支架显示出改善的伤口愈合率、整体闭合和上皮再形成。
Dermal wounds, both acute and chronic, represent a significant clinical challenge and therefore the development of novel biomaterial-based skin substitutes to promote skin repair is essential. Nanofibers have garnered attention as materials to promote skin regeneration due to the similarities in morphology and dimensionality between nanofibers and native extracellular matrix proteins, which are critical in guiding cutaneous wound healing. Electrospun chitosan-poly(caprolactone) (CPCL) nanofiber scaffolds, which combine the important intrinsic biological properties of chitosan and the mechanical integrity and stability of PCL, were evaluated as skin tissue engineering scaffolds using a mouse cutaneous excisional skin defect model. Gross assessment of wound size and measurement of defect recovery over time as well as histological evaluation of wound healing showed that CPCL nanofiber scaffolds increased wound healing rate and promoted more complete wound closure as compared with Tegaderm, a commercially available occlusive dressing. CPCL nanofiber scaffolds represent a biomimetic approach to skin repair by serving as an immediately available provisional matrix to promote wound closure. These nanofiber scaffolds may have significant potential as a skin substitute or as the basis for more complex skin tissue engineering constructs involving integration with biologics. Electrospun chitosan-poly(caprolactone) (CPCL) nanofiber scaffolds showed improved wound healing rate, and overall closure, and re-epithelialization as compared with commercial Tegaderm.