PROPERTIES OF CARCINOGEN ALTERED MOUSE EPIDERMAL-CELLS RESISTANT TO CALCIUM-INDUCED TERMINAL DIFFERENTIATION
PROPERTIES OF CARCINOGEN ALTERED MOUSE EPIDERMAL-CELLS RESISTANT TO CALCIUM-INDUCED TERMINAL DIFFERENTIATION
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DOI：
10.1093/carcin/4.11.1367
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发表时间：
1983-01-01
期刊：
影响因子：
4.7
通讯作者：
YUSPA, SH
中科院分区：
文献类型：
--
作者：
KULESZMARTIN, M;KILKENNY, AE;YUSPA, SH
从原代培养的小鼠表皮细胞中获得了8个对Ca ~（2+）诱导的终末分化具有抗性的细胞系[X_1，A_1，B，X_4，F，G，C，D]，并分析了它们的特性。这些细胞系是自发形成的（2个细胞系），或在原代培养物暴露于致癌物[N-甲基-N“-硝基-N-亚硝基胍或7，12-二甲基苯并[a]蒽]或致癌物和肿瘤促进剂[12-O-十四烷酰基-佛波醇-13-乙酸酯]后形成的。除1个细胞系外，所有细胞系均为上皮或上皮样形态，但8个细胞系中有3个细胞系缺乏桥粒、角蛋白丝和免疫沉淀角蛋白，因此不能被定义为角质形成细胞。在同基因Balb/c新生儿中，5个角质形成细胞系中有2个在4个月后具有致瘤性。在含1.2mM Ca ~（2+）的培养基中，3个细胞系在11个月后仍保持非致瘤性。在1.2mM Ca 2+中。所有3条非角化线都是致瘤性的。5种角质形成细胞系的致瘤潜力与倍性（由DNA含量测定）、转氨酶活性或软琼脂生长无关。这2种致瘤性角质形成细胞系含有对γ-γ射线强红色染色的细胞。谷氨酰转肽酶活性，而非致瘤性角质形成细胞系没有。只有那些缺乏桥粒和角蛋白丝的细胞系在软琼脂中生长，但这些细胞系对γ-谷氨酰转肽酶活性。倍性和转氨酶活性与这些非角化细胞系的致瘤性无关。来源于培养的小鼠表皮细胞的细胞系，并根据其对Ca 2+诱导的终末分化的抗性进行选择，这些细胞系可能是癌前的。这些细胞系中其他标记物与恶性变化的关联取决于细胞是否角化。
Eight cell lines [X1, A1, B, X4, F, G, C, D] exhibiting resistance to Ca2+-induced terminal differentiation were derived from primary mouse epidermal cultures and their properties analyzed. The lines developed either spontaneously (2 lines) or after exposure of primary cultures to carcinogens [N-methyl-N''-nitro-N-nitrosoguanidine or 7,12-dimethylbenz[a]anthracene] or carcinogens and tumor promoter [12-O-tetradecanoyl-phorbol-13-acetate]. All but 1 of the lines were of epithelial or epithelioid morphology but 3 of the 8 lines lacked desmosomes, keratin filaments and immunoprecipitable keratin proteins, and thus could not be defined as keratinocytes. Two of the 5 keratinocyte lines were tumorigenic in syngeneic Balb/c newborns after 4 mo. in medium containing 1.2 mM Ca2+, and 3 lines remained non-tumorigenic even after 11 mo. in 1.2 mM Ca2+. All 3 of the non-keratinizing lines were tumorigenic. Tumorigenic potential of the 5 keratinocyte lines did not correlate with ploidy (as determined by DNA content), transglutaminase activity or growth in soft agar. The 2 tumorigenic keratinocyte lines contained cells which stained intensely red for .gamma.-glutamyl transpeptidase activity, while the non-tumorigenic keratinocyte lines did not. Only those lines lacking desmosomes and keratin filaments grew in soft agar, but these lines were negative for .gamma.-glutamyl transpeptidase activity. Ploidy and transglutaminase activity did not correlate with tumorigenicity in these non-keratinizing lines. Cell lines derived from cultured mouse epidermal cells and selected on the basis of their resistance to Ca2+ induced terminal differentiation may be preneoplastic. The association of additional markers with malignant change in these cell lines depended on whether or not the cells were keratinizing.