Posttranscriptional regulation of ornithine decarboxylase activity.
Posttranscriptional regulation of ornithine decarboxylase activity.
复制标题
鸟氨酸脱羧酶活性的转录后调节。
DOI：
10.1002/jcp.1041260307
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发表时间：
1986
影响因子：
5.6
通讯作者：
Scheffler,IE
中科院分区：
文献类型：
--
作者：
Dircks,L;Grens,A;Slezynger,TC;Scheffler,IE
我们使用了一种过量产生鸟氨酸脱羧酶（ODC）的中国仓鼠卵巢细胞系（DF3），以检查该酶的细胞周期依赖性调控中的各种参数。在各种条件下，ODC活性的改变伴随着蛋白质水平的平行变化，如通过免疫交叉反应物质（CRM）所测量的。虽然腐胺已知抑制ODC的诱导，但我们发现在DF3细胞中10−4M鸟氨酸完全抑制ODC活性。我们还表明，当ODC活性和CRM水平急剧变化时，ODC mRNA水平不受调节。这些数据可以解释的模型，涉及腐胺的ODC mRNA的翻译的影响，或相对特定的蛋白酶与ODC作为其目标的活性。
We have used a Chinese hamster ovary cell line (DF3) that overproduces ornithine decarboxylase (ODC) to examine various parameters in the cell cycle‐dependent regulation of this enzyme. Under a variety of conditions, alterations in the activity of ODC were accompanied by parallel changes in the levels of the protein, as measured by immunologically cross‐reactive material (CRM). While putrescine has been known to suppress the induction of ODC, we have found that in DF3 cells 10−4M ornithine completely suppresses ODC activity. We also show that the levels of ODC mRNA are not modulated when the levels of ODC activity and CRM change drastically. The data can be interpreted in terms of models involving either an effect of putrescine on the translation of ODC mRNA, or on the activity of a relatively specific protease with ODC as its target.