Composition and activity of rhizosphere microbial communities associated with healthy and diseased greenhouse tomatoes
Composition and activity of rhizosphere microbial communities associated with healthy and diseased greenhouse tomatoes
复制标题
与健康和患病温室番茄相关的根际微生物群落的组成和活性
DOI：
10.1007/s11104-014-2097-6
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发表时间：
2014-07-01
期刊：
影响因子：
4.9
通讯作者：
Dong, Yuan-Hua
中科院分区：
文献类型：
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作者：
Li, Jian-Gang;Ren, Gai-Di;Dong, Yuan-Hua
目的研究番茄根际微生物群落的结构和功能潜力。方法采用454焦磷酸测序技术对番茄根际土壤和根部细菌群落的组成变化进行了研究。结果与细菌多样性最高的健康样品相比，患病样品的微生物组成发生了明显的变化。无论是疾病样本还是健康样本，优势类群都是变形杆菌，占种类的35.7-97.4%。革兰氏阳性杆菌类在健康组中的含量高于病变组，而theAlphaproteobacteriaandBetaproteobacteriawere在病变组中的含量较高。病原菌中青枯雷尔斯氏菌和放线杆菌的比例也有所上升。病株与非病株根际土壤和植物根部细菌群落的比例变化趋势相似，说明病原菌侵染改变了植物和土壤中细菌群落的组成。在微生物活性方面，病土样品中的功能多样性受到抑制。土壤酶活性，包括尿素酶、碱性磷酸酶和过氧化氢酶活性也有所下降。在连作番茄作业中，茄子侵染引起微生物群落组成和功能潜力的变化。
AimsThe goal of this study was to investigate the structure and functional potential of microbial communities associated with healthy and diseased tomato rhizospheres.MethodsComposition changes in the bacterial communities inhabiting the rhizospheric soil and roots of tomato plants were detected using 454 pyrosequencing. Microbial functional diversity was investigated with BIOLOG technology.ResultsThere were significant shifts in the microbial composition of diseased samples compared with healthy samples, which had the highest bacterial diversity. The predominant phylum in both diseased and healthy samples wasProteobacteria, which accounted for 35.7–97.4 % of species. The classGammaproteobacteriawas more abundant in healthy than in diseased samples, while theAlphaproteobacteriaandBetaproteobacteriawere more abundant in diseased samples. The proportions of pathogenicRalstonia solanacearumandActinobacteriaspecies were also elevated in diseased samples. The proportions of the various bacterial populations showed a similar trend both in rhizosphere soil and plant roots in diseased versus disease-free samples, indicating that pathogen infection altered the composition of bacterial communities in both plant and soil samples. In terms of microbial activity, functional diversity was suppressed in diseased soil samples. Soil enzyme activity, including urease, alkaline phosphatase and catalase activity, also declined.ConclusionsThis is the first report that provides evidence thatR. solanacearuminfection elicits shifts in the composition and functional potential of microbial communities in a continuous-cropping tomato operation.