Profiling expression patterns of 2,214 unigenes in mouse craniofacial development by cDNA microarray analysis
Profiling expression patterns of 2,214 unigenes in mouse craniofacial development by cDNA microarray analysis
复制标题
通过 cDNA 微阵列分析分析小鼠颅面发育中 2,214 个 unigene 的表达模式
作者：
Haochuan Li;Yidong Chen;Yuan Jiang;M. Bittner;P. Meltzer;J. Trent;Kenneth M. Yamada;Yoshihiko Yamada
近年来，随着基因芯片技术的发展，人们可以同时并行监测数千个基因的表达，为研究基因在不同发育阶段和病理状态下的转录活性提供了有力的工具。我们发起了口腔和颅面基因组解剖计划(OC-GAP)，以确定与颅面发育和疾病有关的重要基因。作为基因组计划的一部分，我们研究了小鼠颅面发育过程中的基因表达模式。将从胚胎第9.5天、13.5天和16.5天的小鼠胚胎头面部组织中提取的mRNA制备成荧光标记的探针，与2,214个小鼠基因印迹的微阵列玻片杂交。基因芯片定量分析表明，其中10%的基因在颅面发育过程中有不同程度的表达，而其余的基因在不同发育阶段的表达没有显著差异。我们发现，41个基因，如编码WNT-1、维甲酸受体和BRCA1的基因，在E9.5时高表达；54个基因，如编码BMP-1、IGF II和SOX3的基因，在E13.5高表达；另外126个基因，如编码Mash-1、FHF-1和Fiblin-2的基因，在E16.5高表达。鉴定这些在头面部发育过程中具有高度阶段特异性表达的基因具有潜在的重要意义，因为它们可能在头面部组织的形成中发挥关键作用。我们目前正在研究E8.5−9.5小鼠头面部基因文库中的基因表达模式，以期通过基因芯片筛选方法鉴定新的头面部基因。
The recent development of cDNA microarray technology enables parallel expression monitoring of thousands of genes simultaneously, which provides a powerful tool for characterizing transcriptional activities of genes in various developmental stages and pathological states. We initiated the Oral and Craniofacial Genome Anatomy Project (OC-GAP) to identify genes important for craniofacial development and disorders. As part of this genome project, we have examined gene expression patterns during mouse craniofacial development. Fluorescently labelled probes prepared using mRNA from mouse embryonic craniofacial tissues at embryonic day (E) 9.5, E13.5 and E16.5 were hybridized to microarray slides printed with 2,214 mouse unigenes. A microarray quantitative analysis revealed that 10% of these genes were expressed in different levels during craniofacial development, whereas the rest showed no significant differences in expression throughout the different developmental stages. We found that 41 genes, such as those encoding Wnt-1, retinoid receptor and Brca1, showed higher expression levels at E9.5; 54 genes, such as those encoding Bmp-1, IGF II and Sox3, were more highly expressed at E13.5; and another 126 genes, such as those encoding Mash 1, FHF-1 and fibulin-2, were expressed at higher levels at E16.5. The identification of these genes with highly stage-specific expression during craniofacial development is potentially important because they are likely to have key roles in the formation of craniofacial tissues. We are currently studying expression patterns of genes from an E8.5−9.5 mouse craniofacial cDNA library to identify novel craniofacial genes by microarray screening approaches.