A Clip Domain Serine Protease Involved in Egg Production in Nilaparvata lugens: Expression Patterns and RNA Interference
A Clip Domain Serine Protease Involved in Egg Production in Nilaparvata lugens: Expression Patterns and RNA Interference
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参与褐飞虱产卵的剪辑域丝氨酸蛋白酶：表达模式和 RNA 干扰
DOI：
10.3390/insects10110378
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发表时间：
2019-11-01
期刊：
影响因子：
3
通讯作者：
Xu, Yi-peng
中科院分区：
文献类型：
--
作者：
Wu, Jia-min;Zheng, Rong-er;Xu, Yi-peng
Clip结构域丝氨酸蛋白酶在各种先天免疫功能和胚胎发育中起重要作用。褐飞虱促凝酶（Nilaparvata lugens proclotting enzymes，NlPCE）属于该蛋白酶家族。据报道，NlPCE 1参与先天免疫，而其他NlPCE的作用尚不清楚。在本研究中，N.克隆并鉴定了褐飞虱前凝血酶3（NlPCE 3）。NlPCE 3含有信号肽、剪辑结构域和胰蛋白酶样丝氨酸蛋白酶结构域。NlPCE 3在检查的所有组织（肠、脂肪体和卵巢）中以及在所有发育阶段中表达。免疫荧光染色显示NlPCE 3主要表达于滤泡细胞的胞浆和胞膜。双链NlPCE 3 RNA干扰明显抑制NlPCE 3的表达，导致卵子形成异常和排卵障碍。这些结果表明，NlPCE 3在黑腹小蜂产卵过程中起重要作用。lugens。
Clip domain serine proteases play vital roles in various innate immune functions and in embryonic development. Nilaparvata lugens proclotting enzymes (NlPCEs) belong to this protease family. NlPCE1 was reported to be involved in innate immunity, whereas the role of other NlPCEs is unclear. In the present study, N. lugens proclotting enzyme-3 (NlPCE3) was cloned and characterized. NlPCE3 contains a signal peptide, a clip domain, and a trypsin-like serine protease domain. NlPCE3 was expressed in all tissues examined (gut, fat body, and ovary), and at all developmental stages. Immunofluorescence staining showed that NlPCE3 was mainly expressed in the cytoplasm and cytomembrane of follicular cells. Double stranded NlPCE3 RNA interference clearly inhibited the expression of NlPCE3, resulting in abnormal egg formation and obstruction of ovulation. These results indicate that NlPCE3 plays an important role in egg production in N. lugens.