Analysis of proteomic responses of freeze-dried Oenococcus oeni to access the molecular mechanism of acid acclimation on cell freeze-drying resistance
Analysis of proteomic responses of freeze-dried Oenococcus oeni to access the molecular mechanism of acid acclimation on cell freeze-drying resistance
复制标题
分析冻干酒球菌的蛋白质组反应，了解酸驯化对细胞冻干抗性的分子机制
DOI：
10.1016/j.foodchem.2019.01.120
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Zhang Guoqiang
中科院分区：
文献类型：
--
作者：
Yang Kun;Zhu Yang;Qi Yiman;Zhang Tingjing;Liu Miaomiao;Zhang Jie;Wei Xinyuan;Fan Mingtao;Zhang Guoqiang
苹果酸-乳酸发酵（Malolactic fermentation，MLF）通常由酒球菌（Oenococcus oeni，O. oeni），是提高葡萄酒质量的重要工艺。酸驯化已被证明是有用的，以提高冻干的活力。oeni。目的：阐明冻干O的作用机制、细胞完整性、形态学和蛋白质图谱。oeniSD-2a进行酸驯化研究。冻干后，酸适应的细胞的完整性和更多的胞外聚合物（EPS）的观察。结合GO和KEGG分析，不同丰度蛋白质在糖类代谢过程中明显富集，尤其是氨基糖和核苷酸糖代谢。最显着的结果是参与细胞壁生物合成、EPS产生、ATP结合和细菌分泌系统的蛋白质的过表达。这一结果表明，酸驯化对细胞被膜特性的重要作用。此外，应激反应蛋白和精氨酸脱亚胺酶途径也被证明是过表达的。
Malolactic fermentation (MLF), usually induced byOenococcus oeni(O. oeni), is an important process to improve wine quality. Acid acclimation has been proven to be useful for enhancing the viability of lyophilizedO. oeni. To explain the involved mechanisms, cell integrity, morphology and protein patterns of lyophilizedO. oeniSD-2a were investigated with acid acclimation. After lyophilization, improvement of cell integrity and more extracellular polymeric substances (EPS) were observed in acid acclimated cells. Combined with GO and KEGG analysis, different abundant proteins were noticeably enriched in the carbohydrate metabolism process, especially amino sugar and nucleotide sugar metabolism. The most significant result was the over-expression of proteins participating in cell wall biosynthesis, EPS production, ATP binding and the bacterial secretion system. This result indicated the important role of acid acclimation on cell envelope properties. In addition, protein response to stress and arginine deiminase pathway were also proven to be over-expressed.