Middle cerebral artery occlusion in the rat by intraluminal suture - Neurological and pathological evaluation of an improved model
Middle cerebral artery occlusion in the rat by intraluminal suture - Neurological and pathological evaluation of an improved model
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DOI：
10.1161/01.str.27.9.1616
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发表时间：
1996-09-01
期刊：
影响因子：
8.3
通讯作者：
Ginsberg, MD
中科院分区：
文献类型：
--
作者：
Belayev, L;Alonso, OF;Ginsberg, MD
Background and Purpose The purpose of the present study was to evaluate a modified method of intraluminal suture occlusion of the middle cerebral artery (MCA) on the volume of brain infarction and on neurobehavioral function in rats subjected to a temporary focal ischemic insult.Methods Male Sprague-Dawley rats were anesthetized with halothane and subjected to 60 minutes or 2 hours of temporary MCA occlusion (MCAo) by an intraluminal thread. In one group of rats, the suture was coated with poly-L-lysine, while in a second group, a conventional uncoated suture was used. Behavioral function was evaluated at 50 to 60 minutes after occlusion and during a 3-day period after MCAo. Three days after MCAo, brains were perfusion-fixed and infarct volumes were measured.Results In rats with 60-minute MCAo, only 3 of 7 animals with uncoated sutures had infarcts, whereas in the group with poly-L-lysine-coated sutures, all rats (n=7) exhibited infarction (P=.009, Fisher's tract test). With 2 hours of MCAo, total infarct volume (corrected for brain edema) was significantly larger in rats with poly-L-lysine-coated sutures than in the group with uncoated sutures (meant SEM, 122.1+/-4.8 versus 67.0+/-18.2 mm(3), respectively; P=.03; n=4 in each group). In the 2-hour MCAo study, infarct volumes in the uncoated-suture group tended to be variable and inconsistent (coefficient of variation, 54%) compared with the group in which sutures were coated with poly-L-lysine, in which a highly consistent infarct was produced (coefficient of variation of infarct volume, 8%).Conclusions Reversible MCAo in which a poly-L-lysine coated intraluminal suture was used proved to be a reliable and effective modification of this technique, yielding consistently larger infarcts and greatly reduced interanimal variability.
