Fully adaptive FEM based fluorescence optical tomography from time-dependent measurements with area illumination and detection
Fully adaptive FEM based fluorescence optical tomography from time-dependent measurements with area illumination and detection
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DOI：
10.1118/1.2190330
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发表时间：
2006-05-01
期刊：
影响因子：
3.8
通讯作者：
Sevick-Muraca, Eva M.
中科院分区：
文献类型：
--
作者：
Joshi, Amit;Bangerth, Wolfgang;Sevick-Muraca, Eva M.
采用区域照明和区域检测方案，我们从具有嵌入式荧光靶的组织体中获得荧光频域测量值，并使用基于自适应有限元的方案获得内部荧光吸收图的层析重建。组织模体为透明丙烯酸立方体盒（512 ml），填充1% Liposyn溶液，荧光靶为直径5mm的玻璃球，填充1% Liposyn中1 μ M吲哚菁绿染料溶液。频域区域照明和检测采用平面激励源，分别采用扩展强度调制（100 MHz） 785 nm二极管激光器和增益调制图像增强电荷耦合器件相机。激发模式的特点是通过交叉偏振片隔离单散射分量，并输入到基于双自适应有限元的方案中，用于嵌入在幻影表面下的荧光目标的三维重建。自适应网格细化技术可以有效地模拟入射激发光，并重建埋在1和2厘米深度的荧光目标。结果证明了第一个临床相关的非接触荧光断层扫描与自适应有限元方法。(c) 2006年美国医学物理学家协会。
Using an area-illumination and area-detection scheme, we acquire fluorescence frequency domain measurements from a tissue phantom with an embedded fluorescent target and obtain tomographic reconstructions of the interior fluorescence absorption map with an adaptive finite element based scheme. The tissue phantom consisted of a clear acrylic cubic box (512 ml) filled with 1% Liposyn solution, while the fluorescent targets were 5 mm diameter glass bulbs filled with 1 mu M Indocyanine Green dye solution in 1% Liposyn. Frequency domain area illumination and detection employed a planar excitation source using an expanded intensity modulated (100 MHz) 785 nm diode laser light and a gain modulated image intensified charge coupled device camera, respectively. The excitation pattern was characterized by isolating the singly scattered component with cross polarizers and was input into a dual adaptive finite element-based scheme for three dimensional reconstructions of fluorescent targets embedded beneath the phantom surface. Adaptive mesh refinement techniques allowed efficient simulation of the incident excitation light and the reconstruction of fluorescent targets buried at the depths of 1 and 2 cm. The results demonstrate the first clinically relevant noncontact fluorescence tomography with adaptive finite element methods. (c) 2006 American Association of Physicists in Medicine.