Polymorphic forms of expressed bovine interferon-τ genes:: Relative transcript abundance during early placental development, promoter sequences of genes and biological activity of protein products
Polymorphic forms of expressed bovine interferon-τ genes:: Relative transcript abundance during early placental development, promoter sequences of genes and biological activity of protein products
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DOI：
10.1210/en.142.7.2906
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发表时间：
2001-07-01
期刊：
影响因子：
4.8
通讯作者：
Roberts, RM
中科院分区：
文献类型：
--
作者：
Ealy, AD;Larson, SF;Roberts, RM
牛中存在多个干扰素（IFN）-tau基因，但仍不清楚有多少表达，其变异程度，以及不同的基因是否表现出相似的表达模式和编码具有相似生物活性的蛋白质。共118个互补DNA（cDNA）的双向测序从反转录牛（bo）孕体RNA从胚泡形成，直到怀孕的第25天。14个不同的cDNA，编码8个不同的IFN-tau，被证实是独特的。全部显示出高序列保守性（> 98%核苷酸同一性; > 96%氨基酸同一性）。cDNA分为三个，最近演变，系统发育组（tau 1，2和9）。第17天和第19天IFN-tau信使RNA的平均浓度高于第14天和第25天，不同的基因显示出相当的表达模式，尽管在囊胚中两种基因（boIFN-tau 1c和tau 3a）的表达似乎存在主要偏倚。克隆了代表三个boIFN-tau组成员的基因。它们的启动子区域相对于被认为对转录激活重要的区域是保守的。三组代表基因在大肠杆菌中产生的重组蛋白具有相似但不完全相同的抗病毒活性。总之，许多IFN-tau基因，这可能是在类似的转录控制下，表达在牛滋养层在围着床期的发展。
Multiple interferon (IFN)-tau genes exist in cattle, but it has remained unclear how many are expressed, the extent of their variation, and whether different genes exhibit similar patterns of expression and code for proteins with similar biological activities. A total of 118 complementary DNA (cDNA) were bi-directionally sequenced from reverse-transcribed bovine (bo) conceptus RNA over the period from blastocyst formation until day 25 of pregnancy. Fourteen different cDNAs, encoding eight different IFN-tau, were confirmed unique. All showed high sequence conservation (> 98% nucleotide identity; > 96% amino acid identity). The cDNA fell into three, recently evolved, phylogenetic groups (tau1, 2, and 9). Mean concentrations of IFN-tau messenger RNA were greater at day 17 and day 19 than at day 14 and day 25, with different genes showing comparable expression patterns, although there appeared to be a major bias in expression of two genes (for boIFN-tau 1c and tau 3a) in blastocysts. Genes representing members of the three boIFN-tau groups were cloned. Their promoter regions were conserved over regions considered important for transcriptional activation. Recombinant protein generated in Escherichia coli from representative genes in the three groups had similar but not identical antiviral activities. In summary, many IFN-tau genes, which are probably under similar transcriptional control, are expressed in bovine trophoblast during the peri-implantation period of development.