Ion Trap with Narrow Aperture Detection Electrodes for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Ion Trap with Narrow Aperture Detection Electrodes for Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
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用于傅里叶变换离子回旋共振质谱分析的具有窄孔径检测电极的离子阱
DOI：
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发表时间：
2015
影响因子：
3.2
通讯作者：
Y. O. Tsybin
中科院分区：
文献类型：
--
作者：
K. Nagornov;Anton N. Kozhinov;O. Tsybin;Y. O. Tsybin
傅里叶变换离子回旋共振质谱（FT-ICR MS）中离子阱（池）的设计目前主要采用宽孔径检测电极的离子检测方法。具体地，激发电极和检测电极通常为90°宽，并且径向地定位在距ICR池轴相似的距离处。在这里，我们证明，离子检测与窄孔径检测电极（NADEL）定位径向向内的细胞的轴是可行的，并有利于FT-ICR MS。我们描述的设计细节和性能特点的10 T FT-ICR MS配备了NADEL ICR细胞具有一对窄孔径（平）检测电极和一对标准的90°激发电极。尽管检测电极的表面积较小，但NADEL ICR单元的灵敏度并未降低，这归因于激发场分布的改善、检测电极的电容的减小以及它们更靠近激发离子的轨道的定位。NADEL ICR池的性能特征与最先进的FT-ICR MS在小分子、肽、蛋白质和石油组学分析中的应用相当。此外，NADEL ICR单元的设计提高了ICR单元的灵活性，并有助于实现高级功能（例如，用于改进主流应用的四极离子检测）。它还为解决FTMS中的主要瓶颈创造了一个有趣的机会-通过同时采集多个瞬态或通过生成周期性非正弦瞬态信号来增加其吞吐量。
图形摘要
AbstractThe current paradigm in ion trap (cell) design for Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) is the ion detection with wide aperture detection electrodes. Specifically, excitation and detection electrodes are typically 90° wide and positioned radially at a similar distance from the ICR cell axis. Here, we demonstrate that ion detection with narrow aperture detection electrodes (NADEL) positioned radially inward of the cell’s axis is feasible and advantageous for FT-ICR MS. We describe design details and performance characteristics of a 10 T FT-ICR MS equipped with a NADEL ICR cell having a pair of narrow aperture (flat) detection electrodes and a pair of standard 90° excitation electrodes. Despite a smaller surface area of the detection electrodes, the sensitivity of the NADEL ICR cell is not reduced attributable to improved excite field distribution, reduced capacitance of the detection electrodes, and their closer positioning to the orbits of excited ions. The performance characteristics of the NADEL ICR cell are comparable with the state-of-the-art FT-ICR MS implementations for small molecule, peptide, protein, and petroleomics analyses. In addition, the NADEL ICR cell’s design improves the flexibility of ICR cells and facilitates implementation of advanced capabilities (e.g., quadrupolar ion detection for improved mainstream applications). It also creates an intriguing opportunity for addressing the major bottleneck in FTMS—increasing its throughput via simultaneous acquisition of multiple transients or via generation of periodic non-sinusoidal transient signals.
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影响因子：
7.4
作者：
Williams, D. Keith, Jr.;Muddiman, David C.
通讯作者：
Muddiman, David C.
半开潘宁阱具有高效的光收集功能，可用于高电荷离子的精密激光光谱分析
影响因子：
--
作者：
David von Lindenfels;Manuel Vogel;Gerhard Birkl;Wolfgang Quint;Marco Wiesel
通讯作者：
Marco Wiesel
影响因子：
7.4
作者：
Kilgour DP;Wills R;Qi Y;O'Connor PB
通讯作者：
O'Connor PB
影响因子：
7.4
作者：
McAlister, Graeme C.;Huttlin, Edward L.;Haas, Wilhelm;Ting, Lily;Jedrychowski, Mark P.;Rogers, John C.;Kuhn, Karsten;Pike, Ian;Grothe, Robert A.;Blethrow, Justin D.;Gygi, Steven P.
通讯作者：
Gygi, Steven P.
影响因子：
7.4
作者：
Weisbrod, Chad R.;Kaiser, Nathan K.;Skulason, Gunnar E.;Bruce, James E.
通讯作者：
Bruce, James E.