A DIET HIGH IN FAT STIMULATES ADIPOCYTE PROLIFERATION IN OLDER (22 MONTH) RATS
A DIET HIGH IN FAT STIMULATES ADIPOCYTE PROLIFERATION IN OLDER (22 MONTH) RATS
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DOI：
10.1016/0531-5565(90)90045-4
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发表时间：
1990-01-01
影响因子：
3.9
通讯作者：
STERN, JS
中科院分区：
文献类型：
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作者：
ELLIS, JR;MCDONALD, RB;STERN, JS
在22月龄雌性Sprague-Dawley大鼠中研究了高脂饮食刺激脂肪细胞增殖的作用，通过将[3 H]-胸苷掺入脂肪细胞DNA进行测定。给大鼠喂食低脂肪（n = 10）或高脂肪饮食（n = 9），共6天。在饮食操作的第4天和第5天，向大鼠注射80 μ Ci/100 g体重的[3 H]-胸苷。大鼠继续在其各自的饮食一天以上，饥饿72小时，然后再喂食储备饮食三周，以增加基质细胞的周转，从而稀释基质DNA的比活性，对脂肪细胞DNA的比活性的影响最小。饮食组在体重、摄食量、宫旁（帕拉）和腹膜后（RP）贮库质量、细胞数量或细胞大小方面无显著差异。在脂肪细胞中，帕拉和RP贮库中的DNA比活性大于基质血管组分。在帕拉和RP库中，喂食高脂肪饲料的大鼠的脂肪细胞比活性显著高于喂食低脂饲料的大鼠。脂肪组织的放射自显影证实，在喂食高脂饮食的大鼠中，有更大百分比的脂肪细胞核被标记。基质细胞中也有少量标记细胞核。总之，当喂食高脂肪饮食时，老年雌性大鼠脂肪细胞增殖增加。
The effect of a high fat diet in stimulating adipocyte proliferation, as measured by the incorporation of [3H]-thymidine into fat cell DNA, was studied in 22-month-old female Sprague-Dawley rats. Rats were fed a low fat (n = 10) or a high fat diet (n = 9) for a total of six days. On days 4 and 5 of dietary manipulation, rats were injected with 80 .mu.Ci/100 g body weight of [3H]-thymidine. Rats were continued on their respective diets for one more day, starved for 72 h and then refed a stock diet for three weeks in order to increase turnover of stroma cells, thus diluting the specific activity of stromal DNA with minimal effect on specific activity of fat cell DNA. The diet groups did not differ significantly with respect to body masses, food intake, parametrial (PARA) and retroperitoneal (RP) depot masses, cell number or cell size. The specific activity of DNA in both PARA and RP depots was greater in the adipocyte than in the stromavascular fraction. Specific activity of fat cells was significantly greater from rats fed the high fat than the low fat diet in both PARA and RP depots. Radioautography of adipose tissue confirmed that there was a greater percentage of adipocyte nuclei labeled in the rats fed the high fat diet. Also, there were few labeled nuclei found in stroma cells. In conclusion, older female rats increased adipocyte proliferation when fed a high fat diet.