Cytoplasmic heat shock granules are formed from precursor particles and are associated with a specific set of mRNAs
Cytoplasmic heat shock granules are formed from precursor particles and are associated with a specific set of mRNAs
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DOI：
10.1128/mcb.9.3.1298-1308.1989
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发表时间：
1989-03
影响因子：
5.3
通讯作者：
L. Nover;K. Scharf;D. Neumann
中科院分区：
文献类型：
--
作者：
L. Nover;K. Scharf;D. Neumann
在热休克番茄细胞培养中，细胞质热休克颗粒(HSGs)与编码未翻译的控制蛋白的mRNAs的特定子集密切相关。这个信使核糖核蛋白复合体在甲醛(约1.30g/cm3)固定后呈CsCl2梯度条带。它含有所有的热休克蛋白和大部分应用于梯度的RNA。在热震过程中，15s前驱体粒子的HSG发生了可逆的聚集。这些前HSG与19年代的植物生殖体并不完全相同。超微结构分析支持HSG的核糖核蛋白性质及其由大约10纳米的前体颗粒组成。一个模型总结了我们的结果。它合理地解释了未翻译的mRNAs在热休克过程中的显著保守性，也可能适用于动物细胞。
In heat-shocked tomato cell cultures, cytoplasmic heat shock granules (HSGs) are tightly associated with a specific subset of mRNAs coding mainly for the untranslated control proteins. This messenger ribonucleoprotein complex was banded in a CsCl gradient after fixation with formaldehyde (approximately 1.30 g/cm3). It contains all the heat shock proteins and most of the RNA applied to the gradient. During heat shock, a reversible aggregation of HSGs from 15S precursor particles can be shown. These pre-HSGs are not identical to the 19S plant prosomes. Ultrastructural analysis supports the ribonucleoprotein nature of HSGs and their composition of approximately 10-nm precursor particles. A model summarizes our results. It gives a reasonable explanation for the striking conservation of untranslated mRNAs during heat shock and may apply also to animal cells.