The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterising the sizes, shapes and interactions of molecules and assemblies in Biochemistry and Polymer Science.
The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterising the sizes, shapes and interactions of molecules and assemblies in Biochemistry and Polymer Science.
复制标题
DOI：
10.1007/s00249-018-1321-3
复制
发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Harding SE
中科院分区：
文献类型：
--
作者：
Harding SE
T. Svedberg现在已经成为生物化学和聚合物科学中一个非常通用和多样化的工具，用于表征颗粒的尺寸，形状和相互作用，尺寸从几纳米到几十微米，或分子量，M（摩尔质量）从几百道尔顿到几百兆道尔顿。我们通过回顾最近的工作来说明这种多样性：（1）用于考古木材保护的M ~ 0.4-0.9 kDa的小木质素样异丁香酚，（2）M = 3.25 kDa的功能性氨基纤维素的蛋白质样缔合，（3）小糖肽抗生素（M ~ 1.5 kDa）及其与抗生素耐药性相关蛋白的相关性（M ~ 47 kDa），（4）破伤风类毒素蛋白TTP（M ~ 150 kDa）和（5）在糖疫苗开发中考虑将TTP掺入两种巨大的糖缀合物中，达到100 MDa。在说明的多样性，我们将突出的发展，在流体动力学分析，使AUC这样一个令人兴奋的和重要的工具，并指出一个潜在的未来发展，其能力扩展到高度集中的系统。
The analytical ultracentrifuge (AUC) invented by T. Svedberg has now become an extremely versatile and diverse tool in Biochemistry and Polymer Science for the characterisation of the sizes, shapes and interactions of particles ranging in size from a few nanometres to tens of microns, or in molecular weight, M (molar mass) terms from a few hundred daltons to hundreds of megadaltons. We illustrate this diversity by reviewing recent work on (1) small lignin-like isoeugenols of M ~ 0.4–0.9 kDa for archaeological wood conservation, (2) protein-like association of a functional amino-cellulose M = 3.25 kDa, (3) a small glycopeptide antibiotic (M ~ 1.5 kDa) and its association with a protein involved in antibiotic resistance (M ~ 47 kDa), (4) tetanus toxoid protein TTP (M ~ 150 kDa) and (5) the incorporation of TTP into two huge glycoconjugates considered in glycovaccine development with molecular weight species in a broad distribution appearing to reach 100 MDa. In illustrating the diversity, we will highlight developments in hydrodynamic analysis which have made the AUC such an exciting and important instrument, and point to a potential future development for extending its capability to highly concentrated systems.
登录
查看更多内容
DOI：
10.1039/jr9480001382
发表时间：
1948-01-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY
影响因子：
--
作者：
CECIL, R;OGSTON, AG
通讯作者：
OGSTON, AG
影响因子：
16.6
作者：
Allison TM;Reading E;Liko I;Baldwin AJ;Laganowsky A;Robinson CV
通讯作者：
Robinson CV
影响因子：
4.6
作者：
Abdelhameed, Ali Saber;Morris, Gordon A.;Harding, Stephen E.
通讯作者：
Harding, Stephen E.
影响因子：
1.8
作者：
McHale, Emily;Braovac, Susan;Kutzke, Hartmut
通讯作者：
Kutzke, Hartmut
影响因子：
11.2
作者：
Abdelhameed, Ali Saber;Morris, Gordon A.;Harding, Stephen E.
通讯作者：
Harding, Stephen E.