Glucose enters mitochondrial metabolism via both carboxylation and decarboxylation of pyruvate in pancreatic islets.
Glucose enters mitochondrial metabolism via both carboxylation and decarboxylation of pyruvate in pancreatic islets.
复制标题
葡萄糖通过胰岛中丙酮酸的羧化和脱羧进入线粒体代谢。
DOI：
10.1016/0026-0495(93)90118-8
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
MacDonald,MJ
中科院分区：
文献类型：
--
作者：
MacDonald,MJ
葡萄糖衍生的丙酮酸代谢进入线粒体途径刺激胰腺 β 细胞中胰岛素释放的途径尚不清楚。 14 CO 2 比率测定用于估计胰岛中通过羧化和脱羧进入柠檬酸循环的葡萄糖衍生的丙酮酸的分数。用葡萄糖估算丙酮酸 14 CO 2 比率，用琥珀酸甲酯估算乙酸 14 CO 2 比率。葡萄糖和琥珀酸甲酯是胰岛素促分泌剂，比丙酮酸和乙酸盐估计的比率更准确，丙酮酸和乙酸盐不会引发胰岛素释放。结果表明，相对等量的丙酮酸通过羧化和脱羧进入柠檬酸循环。
The routes by which glucose-derived pyruvate is metabolized to enter mitochondrial pathways to stimulate insulin release in the pancreatic β cell are unknown. The14CO2ratios assay was used to estimate the fractions of glucose-derived pyruvate that enter the citric acid cycle by carboxylation and decarboxylation in pancreatic islets. Pyruvate14CO2ratios were estimated with glucose, and acetate14CO2ratios were estimated with succinate methyl ester. Glucose and methyl succinate, which are insulin secretagogues, gave more accurate ratios than ratios estimated with pyruvate and acetate, which do not initiate insulin release. The results indicate that relatively equal amounts of pyruvate enter the citric acid cycle by carboxylation and decarboxylation.