Therapeutic levels of erythropoietin (EPO) achieved after gene electrotransfer to skin in mice
Therapeutic levels of erythropoietin (EPO) achieved after gene electrotransfer to skin in mice
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DOI：
10.1038/gt.2010.46
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发表时间：
2010-09-01
期刊：
影响因子：
5.1
通讯作者：
Gehl, J.
中科院分区：
文献类型：
--
作者：
Gothelf, A.;Hojman, P.;Gehl, J.
基因电转移是指通过电穿孔进行基因转染，并且是用于将裸DNA递送到细胞和组织中的有效的非病毒方法。本研究提供了用促红细胞生成素（EPO）基因电转移到小鼠皮肤的数据。9周龄的雌性NMRI小鼠接受一次、两次或三次50 μ g EPO质粒的皮内注射，随后进行电穿孔。用平板电极和100 μ g EPO，血红蛋白显著增加（P
Gene electrotransfer refers to gene transfection by electroporation and is an effective non-viral method for delivering naked DNA into cells and tissues. This study presents data from gene electrotransfer with erythropoietin (EPO) to mouse skin. Nine-week-old female NMRI mice received one, two or three intradermal injections of 50 mu g EPO plasmid and were subsequently electroporated. With plate electrodes and 100 mu g of EPO, a significant increase in hemoglobin (P