Temporal profile of subventricular zone progenitor cell migration following quinolinic acid-induced striatal cell loss
Temporal profile of subventricular zone progenitor cell migration following quinolinic acid-induced striatal cell loss
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DOI：
10.1016/j.neuroscience.2007.03.011
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发表时间：
2007-06-08
期刊：
影响因子：
3.3
通讯作者：
Connor, B.
中科院分区：
文献类型：
--
作者：
Gordon, R. J.;Tattersfield, A. S.;Connor, B.
许多研究表明，神经前体细胞可以直接迁移到脑损伤和疾病的部位，然而，以前还没有确定与损伤诱导和该细胞的迁移反应相关的细胞何时出生的详细检查。因此，这项研究考察了成年大鼠纹状体喹啉酸(QA)损毁后，祖细胞增殖(“出生”)和神经母细胞迁移到受损纹状体的时间相关性。逆转录病毒标记的脑室下区(SVZ)来源的祖细胞表明，QA损伤的纹状体中的细胞丢失增加了祖细胞通过嘴迁移流(RMS)的迁移长达30天。此外，起源于SVZ的一群分裂细胞产生了双重皮质素阳性的神经母细胞，这些细胞迁移到受损的纹状体，以响应QA损伤引起的细胞丢失。溴脱氧尿嘧啶核苷(BrdU)标记的细胞共表达双皮质酮的定量结果显示，损伤纹状体中的大多数细胞是在QA损伤前或损伤后2天内分裂的前体细胞产生的。相反，在QA损伤后2天或更多天，细胞分裂，迁移到纹状体并显示出神经胶质表型。这些结果表明，纹状体QA损伤后，SVZ来源的细胞的定向迁移和神经母细胞分化是急性和短暂的。我们认为这主要是由于随着时间的推移，由于迁移线索的丢失或抑制，新生成的神经母细胞对受损环境做出反应的能力降低。(C)2007年IBRO。爱思唯尔有限公司出版。保留所有权利。
A number of studies have demonstrated directed migration of neural progenitor cells to sites of brain injury and disease, however a detailed examination of when a cell is "born" in relation to injury induction and the migratory response of that cell has not previously been determined. This study therefore examined the temporal correlation between progenitor cell proliferation ("birth") and neuroblast migratory response into the damaged striatum following quinolinic acid (QA) lesioning of the adult rat striatum. Retroviral labeling of subventricular zone (SVZ)-derived progenitor cells demonstrated that cell loss in the QA-lesioned striatum increased progenitor cell migration through the rostral migratory stream (RMS) for up to 30 days. In addition, a population of dividing cells originating from the SVZ generated doublecortin positive neuroblasts that migrated into the damaged striatum in response to cell loss invoked by the QA lesion. Quantification of bromodeoxyuridine (BrdU)-labeled cells co-expressing doublecortin revealed that the majority of cells present in the damaged striatum were generated from progenitor cells dividing within 2 days either prior to or following the QA lesion. In contrast, cells dividing 2 or more days following QA lesioning, migrated into the striatum and exhibited a glial phenotype. These results demonstrate that directed migration of SVZ-derived cells and neuroblast differentiation in response to QA lesioning of the striatum is acute and transient. We propose this is predominantly due to a reduced capacity over time for newly generated neuroblasts to respond to the lesioned environment due to a loss or inhibition of migratory cues. (C) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.