Peroxynitrite rapidly permeates phospholipid membranes
Peroxynitrite rapidly permeates phospholipid membranes
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DOI：
10.1073/pnas.94.26.14243
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发表时间：
1997-12-23
影响因子：
11.1
通讯作者：
Groves, JT
中科院分区：
文献类型：
--
作者：
Marla, SS;Lee, J;Groves, JT
过氧亚硝酸盐(ONOO-)是一种强有力的氧化剂，与许多病理生理过程有关。ONOO-的活性与其自发分解前对生物靶标的可及性有关(t(1/2)约为1 S，pH 7.4，37℃)。以模型磷脂泡体系和锰卟啉为报告分子，我们证明了ONOO-可以自由地穿过磷脂膜。计算得到ONOO-的渗透系数约为8.0×10(-4)cm。S(-1)，与水相当，大约是超氧化物的400倍。我们认为ONOO-是一种重要的生物效应分子，不仅因为它的反应性，而且因为它的高扩散性。
Peroxynitrite (ONOO-) is a potent oxidant implicated in a number of pathophysiological processes. The activity of ONOO- is related to its accessibility to biological targets before its spontaneous decomposition (t(1/2) approximate to 1 s at pH 7.4, 37 degrees C). Using model phospholipid vesicular systems and manganese porphyrins as reporter molecules, we demonstrated that ONOO- freely crosses phospholipid membranes. The calculated permeability coefficient for ONOO- is approximate to 8.0 x 10(-4) cm . s(-1), which compares well with that of water and is approximate to 400 times greater than that of superoxide. We suggest that ONOO- is a significant biological effector molecule not only because of its reactivity but also because of its high diffusibility.