Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants
Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants
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通过限制性内切酶介导的整合（REMI）产生木霉突变体对新烟碱类杀虫剂吡虫啉进行生物降解
DOI：
10.1016/j.chemosphere.2014.01.023
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发表时间：
2014-10-01
期刊：
影响因子：
8.8
通讯作者：
Xu, Shufa
中科院分区：
文献类型：
--
作者：
He, Xiangfeng;Wubie, Abebe Jenberie;Xu, Shufa
REM！采用限制性内切酶介导整合技术，构建了具有降解烟碱类杀虫剂吡虫啉能力的深绿木霉T23突变株。通过PCR和Southern杂交证实了用于在REMI突变体中整合的质粒pBluescript Il KS-hph。在153株转化子中，有57%的转化子对烟碱类杀虫剂吡虫啉的降解能力显著高于野生型菌株T23（p < 0.01）。更具体地，七个单拷贝T.已证实，深绿素123转化体的降解速率比它们的亲本分离物高30%。在所有的转化突变体中，95%的吡虫啉降解率被鉴定为最高的。本研究为提高REMI转化T.深绿色突变体(C)2014爱思唯尔有限公司版权所有。
REM! (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript Il KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p < 0.01). More specifically, seven single-copied T. atroviride 123 transformants have confirmed a 30% higher degradation rate than their parent isolate. Among all transformed mutants, a 95% imidacloprid degradation rate was identified as the highest. This study, thus, provided an effective approach for improving neonicotinoid insecticide-degrading capability using REMI transformed T. atroviride mutants. (C) 2014 Elsevier Ltd. All rights reserved.