Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies.
Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies.
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DOI：
10.1007/978-1-0716-2364-0_10
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发表时间：
2022
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尽管公共卫生不断努力制止或减少吸烟，但吸烟在青年和成年人中仍然很流行。最近电子烟和电子烟产品的使用激增，给公共卫生带来了另一个重大健康挑战。迫切需要使用生理学相关模型来研究吸烟或电子烟对人体健康的影响。据报道，吸烟、吸电子烟或两者兼而有之的人患有支气管炎等呼吸道疾病。在这里，我们将描述从支气管内刷中收集、扩增和培养人气道上皮细胞的方法，并将这些细胞在气液界面暴露于香烟烟雾或电子烟蒸气中。
Despite the continuing public health efforts to stop or reduce smoking, cigarette smoke use remains popular in the youth and adult population. Recent surge in the use of electronic cigarette and vaping products has created another major health challenge in public health. There is an urgent need to use physiologically relevant models to study the health effect of smoking or vaping in human subjects. Airway diseases such as bronchitis have been described in people who smoke, vape or both. Here, we will describe methods to collect, expand and culture human airway epithelial cells from endobronchial brushings, and expose these cells cultured at air-liquid interface to cigarette smoke or electronic cigarette vapor.