Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii
Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii
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DOI：
10.1128/jcm.43.9.4382-4390.2005
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发表时间：
2005-09-01
影响因子：
9.4
通讯作者：
Rodríguez-Valera, F
中科院分区：
文献类型：
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作者：
Bartual, SG;Seifert, H;Rodríguez-Valera, F
在这项研究中，通过使用从 1990 年至 2001 年西班牙和德国医院暴发中回收的 40 个临床鲍曼不动杆菌分离株，以及来自其他欧洲医院的分离株和两个 DSMZ 鲍曼不动杆菌参考菌株，开发和评估了鲍曼不动杆菌的多位点序列分型 (MLST) 方案。为了进行比较，还研究了 Acinetobacter 13 种的两个分离株（sensu Tjernberg 和 Ursing）、两个临床分离株和 A. calcoacetus 的三个 DSMZ 菌株（均属于 A. calcoacetus-A. baumannii 复合体）。为管家基因的保守区域设计了引物，并对所有菌株的七个此类基因（gltA、gyrB、gdhB、recA、cpn60、gpi 和 rpoD）的 305 至 513 bp 内部片段进行了测序。各个位点的等位基因数量从 6 到 12 个不等，在所研究的鲍曼不动杆菌菌株中总共区分了 20 个等位基因谱或序列类型。 MLST 数据与脉冲场凝胶电泳和扩增片段长度多态性指纹分析产生的流行病学分型结果高度一致。 MLST 方案提供了高水平的分辨率和研究鲍曼不动杆菌种群结构和长期流行病学的优秀工具。
In this study a multilocus sequence typing (MLST) scheme for Acinetobacter baumannii was developed and evaluated by using 40 clinical A. baumannii isolates recovered from outbreaks in Spanish and German hospitals during the years 1990 to 2001, as well as isolates from other European hospitals and two DSMZ reference strains of A. baumannii. For comparison, two isolates of Acinetobacter species 13 (sensu Tjernberg and Ursing), two clinical isolates, and three DSMZ strains of A. calcoaceticus (both belonging to the A. calcoaceticus-A. baumannii complex) were also investigated. Primers were designed for conserved regions of housekeeping genes, and 305- to 513-bp internal fragments of seven such genes-gltA, gyrB, gdhB, recA, cpn60, gpi, and rpoD-were sequenced for all strains. The number of alleles at individual loci ranged from 6 to 12, and a total of 20 allelic profiles or sequence types were distinguished among the investigated A. baumannii strains. The MLST data were in high concordance with the epidemiologic typing results generated by pulsed-field gel electrophoresis and amplified fragment length polymorphism fingerprinting. The MLST scheme provides a high level of resolution and an excellent tool for studying the population structure and long-term epidemiology of A. baumannii.