Eggshell Based Nano-Engineered Hydroxyapatite and Poly(lactic) Acid Electrospun Fibers as Potential Tissue Scaffold
Eggshell Based Nano-Engineered Hydroxyapatite and Poly(lactic) Acid Electrospun Fibers as Potential Tissue Scaffold
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DOI：
10.1155/2019/6762575
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发表时间：
2019-01-01
影响因子：
3.1
通讯作者：
Samuel, Temesgen
中科院分区：
文献类型：
--
作者：
Apalangya, Vitus A.;Rangari, Vijaya K.;Samuel, Temesgen
以聚乳酸（PLA）和蛋壳制备的针状羟基磷灰石纳米颗粒为原料制备了纳米复合静电纺丝纤维。x射线衍射谱和扫描电镜显示，羟基磷灰石颗粒高度结晶，形状呈针状，直径为10 ~ 20nm，长度为100 ~ 200nm。采用扫描电镜（SEM）、热重分析（TGA）、动态扫描量热法（DSC）和拉伸测试技术对电纺丝纤维的微观结构、热性能和力学性能进行了表征。扫描电镜研究表明，原始纤维和PLA/EnHA纤维表面都有许多适合细胞附着的孔隙和粗糙边缘。与原始PLA纤维相比，棒状EnHA颗粒的存在增加了PLA纤维的热性能和机械性能。共聚焦光学图像显示，培养48小时后，成骨细胞附着在致密的原始PLA和PLA/HA-10wt%的纤维上。共聚焦染色光学图像显示，孵育96小时后，细胞质延伸分泌连接相邻细胞核。这些结果表明，蛋壳基纳米羟基磷灰石和聚乳酸纤维可能是潜在的组织再生支架。
Nanocomposite electrospun fibers were fabricated from poly(lactic) acid (PLA) and needle-like hydroxyapatite nanoparticles made from eggshells. The X-ray diffraction spectrum and the scanning electron micrograph showed that the hydroxyapatite particles are highly crystalline and are needle-liked in shape with diameters between 10 and 20nm and lengths ranging from 100 to 200nm. The microstructural, thermal, and mechanical properties of the electrospun fibers were characterized using scanning electron microscope (SEM), thermogravimetric analysis (TGA), dynamic scanning calorimetry (DSC), and tensile testing techniques. The SEM study showed that both pristine and PLA/EnHA fibers surfaces exhibited numerous pores and rough edges suitable for cell attachment. The presence of the rod-liked EnHA particles was found to increase thermal and mechanical properties of PLA fibers relative to pristine PLA fibers. The confocal optical images showed that osteoblast cells were found to attach on dense pristine PLA and PLA/HA-10wt% fibers after 48 hours of incubation. The stained confocal optical images indicated the secretion of cytoplasmic extension linking adjoining nuclei after 96 hours of incubation. These findings showed that eggshell based nanohydroxyapatite and poly(lactic acid) fibers could be potential scaffold for tissue regeneration.