Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients
Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients
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DOI：
10.1111/j.1600-0463.2011.02726.x
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发表时间：
2011-04-01
期刊：
影响因子：
2.8
通讯作者：
Ciofu, Oana
中科院分区：
文献类型：
--
作者：
Lee, Baoleri;Schjerling, Charlotte K.;Ciofu, Oana
囊性纤维化患者呼吸道中铜绿假单胞菌的表型和基因多样性促进了慢性肺部感染期间细菌的长期存活。12个克隆性相关、顺序粘液性和非粘液性配对铜绿假单胞菌从3名丹麦CF患者中获得。在静态和流过条件下研究了生物膜的体外形成能力，并利用Affymetrix基因芯片研究了整体基因表达谱。对藻酸盐产生和群体感应(QS)系统的调控基因进行了测序，并进行了藻酸盐产生的测量和QS信号分子的检测。对感染早期和晚期的粘液型和非粘液型分离株的比较表明，粘液型表型保持了10年以上形成体外生物膜的能力，并显示出没有改变的转录谱，而相应的非粘液型分离物的转录谱发生了显著变化，失去了体外生物膜的形成能力。在这个特殊的铜绿假单胞菌克隆中观察到的粘液分离株中保守的基因表达模式与非粘液分离株中变化的多样性反映了这两种表型在不同的肺环境中所使用的不同的适应策略。
Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated. The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed. Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype. The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.