Expression of multiple tau isoforms and microtubule bundle formation in fibroblasts transfected with a single tau cDNA.
Expression of multiple tau isoforms and microtubule bundle formation in fibroblasts transfected with a single tau cDNA.
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DOI：
10.1083/jcb.109.3.1173
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发表时间：
1989-09
期刊：
影响因子：
--
通讯作者：
Hirokawa N
中科院分区：
文献类型：
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作者：
Kanai Y;Takemura R;Oshima T;Mori H;Ihara Y;Yanagisawa M;Masaki T;Hirokawa N
Tau蛋白是一类低分子质量的微管相关蛋白，在神经系统中特异表达。分离并测序了成年大鼠tau基因的cDNA克隆。为了分析tau蛋白在体内的功能，我们进行了转基因实验。在成纤维细胞系中表达3条tau蛋白，而在大鼠脑内表达6条tau蛋白。去磷酸化后，三条带中的一条消失，直接证明磷酸化参与了tau的多样性。在形态上，我们观察到在瞬时和稳定的tau基因转染组细胞中形成了厚厚的微管束。此外，我们还发现，在稳定表达的细胞中，微管蛋白的产生显著增加。因此，我们推测，tau蛋白促进微管蛋白的聚合，在体内形成微管束，在神经细胞突起的生长和维持中发挥重要作用。
Tau proteins are a class of low molecular mass microtubule-associated proteins that are specifically expressed in the nervous system. A cDNA clone of adult rat tau was isolated and sequenced. To analyze functions of tau proteins in vivo, we carried out transfection experiments. A fibroblast cell line, which was transfected with the cDNA, expressed three bands of tau, while six bands were expressed in rat brain. After dephosphorylation, one of the three bands disappeared, demonstrating directly that phosphorylation was involved in the multiplicity of tau. Morphologically, we observed a thick bundle formation of microtubules in the transiently and stably tau-gene-transfected cells. In addition, we found that the production of tubulin was prominently enhanced in the stably transfected cells. Thus, we suppose that tau proteins promote polymerization of tubulin, form bundles of microtubules in vivo, and play important roles in growing and maintaining nerve cell processes.