The Distribution of 15-Hydroxy Prosta-glandin Dehydrogenase and Prostaglandin-Δ13-Reductase in Tissues of the Swine
The Distribution of 15-Hydroxy Prosta-glandin Dehydrogenase and Prostaglandin-Δ13-Reductase in Tissues of the Swine
复制标题
15-羟基前列腺素脱氢酶和前列腺素-Δ13-还原酶在猪组织中的分布
DOI：
--
复制
发表时间：
1971
期刊：
影响因子：
--
通讯作者：
B. Samuelsson
中科院分区：
文献类型：
--
作者：
Erik Änggård;C. Larsson;B. Samuelsson
在猪的 15 个不同组织的匀浆中测定了前列腺素脱氢酶（15-羟基前列腺酸氧化还原酶，EC 1.1.1 (PGDH) 和前列腺素-Δ13-还原酶（前列腺素 Δ13-氧化还原酶）。这两种酶主要位于无颗粒部分。PGDH 的最高活性出现在肺、脾和肾中，范围为在胃、睾丸、肝脏和小肠中观察到 PGDH 的活性较低，为 50-82 mU/g 组织或从 482 mU/g 到 808 mU/g 蛋白质。肾皮质中的 PGDH 含量约为髓质的三倍，在脾、肾、肝脏、肾上腺和小肠中含量最高，范围为 27-38 mU/g 组织或 232-390。然而，在含有 3383 mU/g 蛋白质的脂肪组织中发现了最高的比活性。这些酶的广泛分布以及其他证据支持它们催化前列腺素代谢中的初始反应的概念。
Prostaglandin dehydrogenase (15-hydroxy prostanoate oxidoreductase, EC 1.1.1 (PGDH) and prostaglandin-Δ13-reductase (prostanoate Δ13-oxidoreductase) were assayed in homogenates of 15 different tissues of the swine. Both enzymes were located mainly in the particle free fraction. The highest activities for PGDH were found in the lung, spleen and kidney, which ranged from 50–82 mU/g of tissue or from 482 to 808 m/g of protein. Lower activities of PGDH were observed in the stomach, testicle, liver and small intestine. The renal cortex contained about three times more PGDH than the medulla. The reductase was most abundant in spleen, kidney, liver, adrenal and small intestine, which ranged between 27–38 mU/g of tissue or 232–390 mU/g of protein. The highest specific activity was however found in adipose tissue which contained 3383 mU/g of protein. The wide distribution of these enzymes as well as other evidence support the concept that they catalyze the initial reactions in the metbolism of the prostaglandins.