Characterization of the Cell-Nanopillar Interface by Transmission Electron Microscopy
Characterization of the Cell-Nanopillar Interface by Transmission Electron Microscopy
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DOI：
10.1021/nl303163y
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发表时间：
2012-11-01
期刊：
影响因子：
10.8
通讯作者：
Cui, Bianxiao
中科院分区：
文献类型：
--
作者：
Hanson, Lindsey;Lin, Ziliang Carter;Cui, Bianxiao
垂直排列的纳米柱可以用作优异的电学、光学。机械平台。用于生物学研究。然而，揭示细胞和纳米柱之间界面的性质是非常具有挑战性的。特别是，一个争论的问题是细胞膜是否在纳米柱周围保持完整。在这里，我们提出了一个详细的表征细胞纳米柱界面的透射电子显微镜。我们研究了生长在直径为50-500 nm，高度为0.5-2 μ m的纳米柱上的皮质神经元。我们发现，在直径小于300 μ m的纳米柱上，细胞膜包裹在整个纳米柱周围，而纳米柱没有渗透到细胞内部。另一方面，细胞坐着。在更大的密集纳米柱阵列之上。我们还观察到，纳米柱的细胞体和神经突的膜表面间隙比平坦基底的小。这些结果支持细胞膜和纳米柱之间的紧密相互作用以及先前使用纳米柱电极在电生理记录中的优异密封的发现。
Vertically aligned nanopillars can serve as excellent electrical, optical. and mechanical platforms. for biological studies. However, revealing the nature of the interface between the cell and the nanopillar is Very challenging. In particular, a matter of debate is whether the cell Membrane remains intact around the nanopillar. Here we present a detailed characterization of the cell-nanopillar interface by transmission electron microscopy.. We examined cortical neurons growing on nanopillars with diameter 50-500 nm and heights 0.5-2 mu m. We found that on nanopillars, less than 300 am in diameter, the cell Membrane wraps around the entirety of the nanopillar Without the nanopillar penetrating into the interior of the cell. On the other hand, the cell sits. on top of arrays of larger, closely Spaced nanopillars. We also observed that the membrane-surface gap Of both cell bodies and neurites is smaller for nanopillars than for a flat substrate. These results support a tight interaction between the cell membrane and the nanopillars and previous findings of excellent sealing in electrophysiology recordings using nanopillar electrodes.