ESTABLISHMENT OF A CONTINUOUS TUMOR-CELL LINE (PANC-1) FROM A HUMAN CARCINOMA OF EXOCRINE PANCREAS
ESTABLISHMENT OF A CONTINUOUS TUMOR-CELL LINE (PANC-1) FROM A HUMAN CARCINOMA OF EXOCRINE PANCREAS
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DOI：
10.1002/ijc.2910150505
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发表时间：
1975-01-01
影响因子：
6.4
通讯作者：
TODARO, G
中科院分区：
文献类型：
--
作者：
LIEBER, M;MAZZETTA, J;TODARO, G
上皮样细胞系，从人胰腺癌的导管细胞起源，已保持在培养物中超过2年，并已传代培养超过40次。PANC-1细胞系具有52 h的倍增时间和慢迁移率或B型的G6 PD活性。染色体研究显示众数为63，有三条不同的标记染色体和一条小的环状染色体。PANC-1细胞系的恶性性质通过以下方式得到验证：（1）PANC-1细胞在软琼脂中和成纤维细胞单层上快速生长;和（2）向裸鼠注射PANC-1细胞后形成进行性生长的间变性癌。
An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC‐1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility or B type. Chromosome studies show a modal number of 63 with three distinct marker chromosomes and a small ring chromosome. The malignant nature of the PANC‐1 cell line was verified by: (1) the ready growth of PANC‐1 cells in soft agar and on top of a fibroblast monolayer; and (2) the formation of a progressively growing anaplastic carcinoma after injection of a nude‐athymic mouse with PANC‐1 cells.