Zinc Oxide Nanoparticles Influence Microflora in Ileal Digesta and Correlate Well with Blood Metabolites.
Zinc Oxide Nanoparticles Influence Microflora in Ileal Digesta and Correlate Well with Blood Metabolites.
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DOI：
10.3389/fmicb.2017.00992
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发表时间：
2017
影响因子：
5.2
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Feng Y;Min L;Zhang W;Liu J;Hou Z;Chu M;Li L;Shen W;Zhao Y;Zhang H
氧化锌纳米颗粒（ZnO NPs）广泛应用于消费品和工业产品中，然而，它们对肠道微生物群和代谢的影响以及它们之间的相互作用尚未完全了解。在这项研究中，氧化锌纳米颗粒对回肠细菌群落，血浆代谢产物的影响，以及它们之间的相关性进行了研究。用不同浓度的ZnO NPs [基于Zn; 0 mg/kg（对照）、25 mg/kg、50 mg/kg和100 mg/kg]饲喂母鸡9周。随后，收集回肠回肠和血浆，分别用于微生物菌群和代谢物的分析。使用Illumina HiSeq 2500平台对回肠肠杆菌微生物群的16 S rRNA基因的V3-V4区域进行测序。回肠中的主要细菌群落属于厚壁菌门。细菌群落的丰富度与ZnO纳米粒子添加量呈负相关（r =-0.636，P < 0.01），当ZnO纳米粒子添加量为100 mg/kg时，微生物多样性显著降低（P < 0.05）。LEfSe分析表明，ZnO纳米颗粒改变了细菌、梭菌和变形菌的群落结构，减少了乳酸菌的数量。此外，通过核磁共振（NMR）分析的代谢表明，葡萄糖、一些氨基酸和其他代谢物被ZnO NPs改变。胆碱、乳酸和蛋氨酸与细菌丰富度呈正相关。总之，ZnO纳米颗粒可以影响回肠回肠中微生物群落的水平，特别是乳酸杆菌。此外，微生物群的丰富程度与胆碱、乳酸和蛋氨酸代谢的变化有关。
Zinc oxide nanoparticles (ZnO NPs) are used widely in consumer and industrial products, however, their influence on gut microbiota and metabolism and their mutual interactions are not fully understood. In this study, the effects of ZnO NPs on ileal bacterial communities, plasma metabolites, and correlations between them were investigated. Hens were fed with different concentrations of ZnO NPs [based on Zn; 0 mg/kg (control), 25 mg/kg, 50 mg/kg, and 100 mg/kg] for 9 weeks. Subsequently, ileal digesta and blood plasma were collected for analysis of microflora and metabolites, respectively. The V3-V4 region of the 16S rRNA gene of ileal digesta microbiota was sequenced using the Illumina HiSeq 2500 platform. The predominant bacterial community in the ileum belongs to the phylum Firmicutes. The richness of the bacterial community was negatively correlated with increasing amounts of ZnO NPs (r = -0.636, P < 0.01); when ZnO NP levels were at 100 mg/kg, microbiota diversity was significantly decreased (P < 0.05). The community structure determined by LEfSe analysis indicated that Bacilli, Fusobacteria, and Proteobacteria were changed, and Lactobacillus was reduced by ZnO NPs. Moreover, metabolism as analyzed by nuclear magnetic resonance (NMR) indicated that glucose, some amino acids, and other metabolites were changed by ZnO NPs. Choline, lactate, and methionine were positively correlated with bacterial richness. In summary, ZnO NPs could influence the levels of microflora in ileal digesta, particularly Lactobacillus. Furthermore, the richness of the microbiota was related to changes in choline, lactate, and methionine metabolism.