Antigenic variation of TprK V regions abrogates specific antibody binding in syphilis
Antigenic variation of TprK V regions abrogates specific antibody binding in syphilis
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DOI：
10.1128/iai.00827-06
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发表时间：
2006-11-01
影响因子：
3.1
通讯作者：
Lukehart, Sheila A.
中科院分区：
文献类型：
--
作者：
LaFond, Rebecca E.;Molini, Barbara J.;Lukehart, Sheila A.
梅毒螺旋体（Treponema pallidum subsp.苍白球在七个可变（V）区域中经历抗原变异。tprK在T. pallidum菌株，并且已经开发了一种方法来获得T.苍白球表达一个单一的，独特的tprK序列。用三种不同的T. pallidum克隆或衍生该克隆的亲本菌株，并通过免疫测定法检查它们的血清对代表来自每个克隆的TprKV区的合成肽的抗体反应性。亲本菌株表达许多不同的V区序列，并且用该菌株感染诱导针对多种V区的抗体应答。在感染芝加哥C克隆的兔子中，产生了针对除V1以外的所有V区的抗体，而在感染芝加哥A克隆的兔子中，产生了仅针对V5、V6和V7的抗体。在芝加哥B感染期间，产生了针对除V1和V3之外的所有V区的抗体。抗体对感染克隆的V区具有高度特异性，交叉反应性很少见。V区引起变体特异性抗体应答的证明支持了TprK变体可能帮助生物体避免感染个体中发生免疫应答的假设，有助于T.苍白球，以建立慢性感染。
The tprK gene in the syphilis spirochete, Treponema pallidum subsp. pallidum, undergoes antigenic variation in seven variable (V) regions. tprK is highly variable within T. pallidum strains, and a method has been developed to derive clones of T. pallidum that express a single, unique tprK sequence. Rabbits were infected with three different T. pallidum clones or the parent strain from which the clones were derived, and their sera were examined by immunoassay for antibody reactivity against synthetic peptides representing the TprK V regions from each clone. The parent strain expresses many different V region sequences, and infection with this strain induced antibody responses against a wide variety of V regions. In rabbits infected with the Chicago C clone, antibodies developed against all of the V regions except V1, while antibodies developed against only V5, V6, and V7 in Chicago A-infected rabbits. During Chicago B infection, antibodies developed against all of the V regions except V1 and V3. Antibodies were highly specific for the V regions of the infecting clone, and cross-reactivity was rare. The demonstration that the V regions elicit a variant-specific antibody response supports the hypothesis that TprK variants may help organisms to avoid the developing immune response in infected individuals, contributing to the ability of T. pallidum to establish chronic infection.