Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33
Variation of microbial rhizosphere communities in response to crop species, soil origin, and inoculation with Sinorhizobium meliloti L33
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DOI：
10.1007/s002480000021
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发表时间：
2000-07-01
影响因子：
3.6
通讯作者：
Tebbe, CC
中科院分区：
文献类型：
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作者：
Miethling, R;Wieland, G;Tebbe, CC
进行了温室研究，以比较作物种类，土壤来源，和细菌接种剂对建立微生物群落定殖植物根部的影响。两种作物，苜蓿（紫花苜蓿）和黑麦（黑麦），分别生长在两个土壤中收集农田在不同的位置和不同的历史豆科作物轮作。将荧光素酶标记基因滞后的苜蓿中华根瘤菌L33（Sinorhizobium meliloti strain L33）以10（6）cfu g（-1）土壤接种到一个小宇宙亚群中。sativa.在培养10周后从苜蓿根际收集微生物聚生体，在培养11周后从黑麦根际收集微生物聚生体。S.苜蓿L33种群在苜蓿根际的数量比黑麦高1 ~ 2个数量级。在种植过苜蓿的土壤中，80%的苜蓿根瘤被土著细菌定殖，而在另一种土壤中，苜蓿根瘤几乎全部被S.苜蓿L33。采用3种群落水平的靶向方法对提取的根际微生物菌群的变异进行了表征：（1）群落水平的生理概况（CLPP）;（2）脂肪酸甲酯分析（FAME）;（3）温度梯度凝胶电泳（TGGE）测定的直接提取的核糖体PCR扩增的16 S rRNA靶序列的多样性。所有的方法都确定了作物种类作为微生物群落特征的主要决定因素。因此，土壤的影响是次要的，而与苜蓿相关的微生物群落结构的改变接种S。苜蓿L33仅通过TGGE一致地观察到。
A greenhouse study with soil-plant microcosms was conducted in order to compare the effect of crop species, soil origin, and a bacterial inoculant on the establishment of microbial communities colonizing plant roots. Two crop species, alfalfa (Medicago sativa) and rye (Secale cereale), were grown separately in two soils collected from agricultural fields at different locations and with differing histories of leguminous crop rotation. A subset of microcosms was inoculated at 10(6) cfu g(-1) soil with the luciferase marker gene-lagged Sinorhizobium meliloti strain L33, a symbiotic partner of M. sativa. Microbial consortia were collected from the rhizospheres of alfalfa after 10 weeks of incubation and from rye after 11 weeks. S. meliloti L33 populations were one to two orders of magnitude higher in the rhizospheres of alfalfa than of rye. In soil with previous alfalfa cultivation, 80% of the alfalfa nodules were colonized by indigenous bacteria, while in the other soil alfalfa was colonized almost exclusively (>90%) with S. meliloti L33. Three community-level targeting approaches were used to characterize the variation of the extracted microbial rhizosphere consortia: (1) Community level physiological profiles (CLPP), (2) fatty acid methyl ester analysis (FAME), and (3) diversity of PCR amplified 16S rRNA target sequences from directly extracted ribosomes, determined by temperature gradient gel electrophoresis (TGGE). All approaches identified the crop species as the major determinant of microbial community characteristics. Consistently, the influence of soil was of minor importance, while a modification of the alfalfa-associated microbial community structure after inoculation with S. meliloti L33 was only consistently observed by using TGGE.