PRODUCTION AND CHARACTERIZATION OF BOVINE MONOCLONAL-ANTIBODIES TO RESPIRATORY SYNCYTIAL VIRUS
PRODUCTION AND CHARACTERIZATION OF BOVINE MONOCLONAL-ANTIBODIES TO RESPIRATORY SYNCYTIAL VIRUS
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DOI：
10.1099/0022-1317-69-12-3023
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发表时间：
1988-12-01
影响因子：
3.8
通讯作者：
STOTT, EJ
中科院分区：
文献类型：
--
作者：
KENNEDY, HE;JONES, BV;STOTT, EJ
产生了六种分泌抗呼吸道合胞（RS）病毒的牛单克隆抗体（MAb）的种间杂交瘤（异种杂交瘤）。使用小鼠骨髓瘤细胞系NS1作为融合配偶体形成四个异源杂交瘤，一个使用NS0形成，剩余的异源杂交瘤使用牛x细胞形成。鼠杂交瘤作为融合伴侣。 5 个异源杂交瘤分泌牛 IgG1，1 个异源杂交瘤分泌 IgG2。所有六种单克隆抗体均可识别人类 A 亚型和 B 亚型病毒以及牛 RS 病毒。它们对融合糖蛋白具有特异性，并在天然抗原的蛋白质印迹中与 140K 二聚体和 70K 单体发生反应；三个还与还原抗原印迹中的 46K F1 成分及其 22K 裂解产物结合。其中两种 MAb 中和了 RS 病毒的感染性，抑制了病毒诱导的融合，在补体存在的情况下裂解了 RS 病毒感染的细胞，并保护小鼠免受 RS 病毒的攻击。
Six interspecific hybridomas (heterohybridomas) secreting bovine monoclonal antibodies (MAbs) against respiratory syncytial (RS) virus were produced. Four of the heterohybridomas were formed using the mouse myeloma cell line NS1 as the fusion partner, one using NS0, and the remaining heterohybridoma was formed using a bovine .times. murine hybridoma as the fusion partner. Five heterohybridomas secreted bovine IgG1 and one secreted IgG2. All six MAbs recognized human subtype A and B viruses as well as bovine RS virus. They were specific for the fusion glycoprotein and reacted with a 140K dimer and a 70K monomer in a Western blot of native antigen; three also bound to the 46K F1 component and its 22K cleavage product in a blot of reduced antigen. Two of these MAbs neutralized RS virus infectivity, inhibited virus-induced fusion, lysed RS virus-infected cells in the presence of complement and protected mice against RS virus challenge.