Rapid identification of bacteria with miniaturized pyrolysis/GC analysis
Rapid identification of bacteria with miniaturized pyrolysis/GC analysis
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通过小型热解/GC 分析快速鉴定细菌
DOI：
10.1117/12.417450
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
P. Lewis
中科院分区：
文献类型：
--
作者：
C. H. Morgan;C. Mowry;R. Manginell;G. Frye;R. Kottenstette;P. Lewis
细菌和其他生物部分的鉴定在环境、生物医学、农业、工业和军事领域有广泛的应用。连接这些应用的是生物标记物，如脂肪酸，其质谱图可用于表征生物样品，并在克型，属，甚至物种水平上区分细菌。常见的样品分析方法需要既冗长又劳动密集的样品制备，特别是对于全细胞细菌。本文所依赖的背景技术利用脂肪酸化学衍生为更挥发性的脂肪酸甲酯（FAME），其可以在气相色谱柱上分离或直接输入质谱仪。最近的出版物表明，在入口处使用热解对整个细菌样品进行原位衍生化可以改善样品制备时间;尽管比传统技术快得多，但这些系统仍然依赖于台式分析设备和单独的样品制备。该小组开发的小型化热解/GC仪器旨在实现细菌和其他生物样品FAME鉴定的好处，同时进一步促进样品处理和仪器便携性。正在制造和测试的技术具有在非常小的规模上实现热解和FAME分离的潜力，具有快速检测时间（从引入到结果的1-10分钟），并且具有模块化样品入口。性能结果和传感器表征将提交的仪器开发的第一阶段，包括微加工热解和气相色谱仪的元素。
Identification of bacteria and other biological moieties finds a broad range of applications in the environmental, biomedical, agricultural, industrial, and military arenas. Linking these applications are biological markers such as fatty acids, whose mass spectral profiles can be used to characterize biological samples and to distinguish bacteria at the gram-type, genera, and even species level. Common methods of sample analysis require sample preparation that is both lengthy and labor intensive, especially for whole cell bacteria. The background technique relied on here utilizes chemical derivatization of fatty acids to the more volatile fatty acid methyl esters (FAMEs), which can be separated on a gas chromatograph column or input directly into a mass spectrometer. More recent publications demonstrate improved sample preparation time with in situ derivatization of whole bacterial samples using pyrolysis at the inlet; although much faster than traditional techniques, these systems still rely on bench-top analytical equipment and individual sample preparation. Development of a miniaturized pyrolysis/GC instrument by this group is intended to realize the benefits of FAME identification of bacteria and other biological samples while further facilitating sample handling and instrument portability. The technologies being fabricated and tested have the potential of achieving pyrolysis and FAME separation on a very small scale, with rapid detection time (1-10 min from introduction to result), and with a modular sample inlet. Performance results and sensor characterization will be presented for the first phase of instrument development, encompassing the microfabricated pyrolysis and gas chromatograph elements.