Glutamine stimulates mTORC1 independent of the cell content of essential amino acids
Glutamine stimulates mTORC1 independent of the cell content of essential amino acids
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DOI：
10.1007/s00726-012-1312-0
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发表时间：
2012-12-01
期刊：
影响因子：
3.5
通讯作者：
Bussolati, Ovidio
中科院分区：
文献类型：
--
作者：
Chiu, Martina;Tardito, Saverio;Bussolati, Ovidio
谷氨酰胺和亮氨酸是重要的 mTORC1 调节剂，尽管它们的作用尚未明确定义。在 HepG2 和 HeLa 细胞中，无谷氨酰胺孵育会降低 mTORC1 活性，但细胞亮氨酸并未降低。 mTORC1 活性受到无氨基酸孵育的抑制，只有同时恢复必需氨基酸 (EAA) 和谷氨酰胺时才能完全恢复，尽管细胞亮氨酸在缺乏谷氨酰胺的情况下要高于存在谷氨酰胺的情况。因此，谷氨酰胺独立于细胞亮氨酸刺激 mTORC1，表明存在来自谷氨酰胺或 EAA 的两种不同的刺激信号。
Glutamine and leucine are important mTORC1 modulators, although their roles are not precisely defined. In HepG2 and HeLa cells glutamine-free incubation lowers mTORC1 activity, although cell leucine is not decreased. mTORC1 activity, suppressed by amino acid-free incubation, is completely rescued only if essential amino acids (EAA) and glutamine are simultaneously restored, although cell leucine is higher in the absence than in the presence of glutamine. Thus, glutamine stimulates mTORC1 independent of cell leucine, suggesting the existence of two distinct stimulatory signals from either glutamine or EAA.