Red-blood-cell-membrane-enveloped magnetic nanoclusters as a biomimetic theranostic nanoplatform for bimodal imaging-guided cancer photothermal therapy
Red-blood-cell-membrane-enveloped magnetic nanoclusters as a biomimetic theranostic nanoplatform for bimodal imaging-guided cancer photothermal therapy
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红细胞膜包裹的磁性纳米团簇作为双模成像引导癌症光热治疗的仿生治疗诊断纳米平台。
DOI：
10.1039/c9tb01829h
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发表时间：
2020-01-28
影响因子：
7
通讯作者：
Feng, Liangzhu
中科院分区：
文献类型：
--
作者：
Wang, Sheng;Yin, Yipengchen;Feng, Liangzhu
红细胞（RBC）膜涂层的使用最近被发现是一种仿生策略，通过利用红细胞固有的长血液循环时间，赋予内核纳米材料改善的药代动力学特征。在这里，我们将超顺磁性纳米团簇（MNCs）包裹在RBC膜上，得到MNC@RBCs，与裸MNCs相比，其生理稳定性显著提高。在负载近红外（NIR） cypate分子后，制备的Cyp-MNC@RBCs具有显著提高的近红外吸光度和光热转换效率。通过在体内荧光成像系统中跟踪cypate的近红外荧光，我们发现，与裸加载cypate的MNCs相比，静脉注射这种Cyp-MNC@RBCs具有显着提高的肿瘤归巢能力。通过记录跨国公司的t2加权磁共振成像（MRI）信号，进一步证明了类似的结果。此外，在808 nm激光照射下，静脉注射Cyp-MNC@RBCs小鼠的肿瘤比单纯注射MNC@RBCs小鼠的肿瘤表现出更高的温度升高，因此通过光热消融可以明显抑制肿瘤生长。本研究提出了制备具有极大改善肿瘤归家能力的仿生Cyp-MNC@RBCs作为荧光和MRI双峰成像引导癌症光热治疗的多功能纳米治疗剂。
The use of red blood cell (RBC) membrane coatings has recently been found to be a biomimetic strategy to confer inner core nanomaterials with improved pharmacokinetic profiles by utilizing the intrinsic long blood circulation time of RBCs. Here, we envelope superparamagnetic nanoclusters (MNCs) with RBC membrane ghosts to obtain MNC@RBCs with significantly improved physiological stability compared to that of bare MNCs. After being loaded with near-infrared (NIR) cypate molecules, the as-prepared Cyp-MNC@RBCs show remarkably increased NIR absorbance and resultant efficient photothermal conversion efficacy. By tracking the NIR fluorescence of cypate in an in vivo fluorescence imaging system, we uncover that such Cyp-MNC@RBCs upon intravenous injection show significantly improved tumor-homing capacity as compared to bare cypate-loaded MNCs. A similar result is further evidenced by recording the T2-weighted magnetic resonance imaging (MRI) signal of MNCs. Furthermore, upon exposure to 808 nm laser irradiation, the tumors grown on the mice with the intravenous injection of Cyp-MNC@RBCs show a higher temperature increase than the tumors grown on the mice injected with plain MNC@RBCs and thus are significantly suppressed via photothermal ablation. This study presents the preparation of biomimetic Cyp-MNC@RBCs with greatly improved tumor-homing capacity as multifunctional nanotheranostic agents for fluorescence and MRI bimodal imaging-guided cancer photothermal therapy.