Grain Sorghum Proteomics: Integrated Approach toward Characterization of Endosperm Storage Proteins in Kafirin Allelic Variants
Grain Sorghum Proteomics: Integrated Approach toward Characterization of Endosperm Storage Proteins in Kafirin Allelic Variants
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DOI：
10.1021/jf5022847
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发表时间：
2014-10-08
影响因子：
6.1
通讯作者：
Godwin, Ian D.
中科院分区：
文献类型：
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作者：
Cremer, Julia E.;Bean, Scott R.;Godwin, Ian D.
谷物蛋白质组成决定了品质性状，如食品、饲料和生物材料用途的价值。高粱中的主要贮藏蛋白是醇溶谷蛋白，称为高粱醇溶蛋白。主要位于淀粉周围的蛋白质体的外围，富含半胱氨酸的β-和γ-酪蛋白酶可限制酶促接近内部定位的α-酪蛋白酶和淀粉。一个综合的方法被用来表征高粱与kafirin位点的等位基因变异，以确定这种遗传多样性对蛋白质表达的影响。反相高效液相色谱和芯片实验室分析。显示β-酪蛋白空株中醇溶性蛋白减少。凝胶分离与液相色谱串联。质谱鉴定了一系列影响贮藏蛋白生物化学的氧化还原活性蛋白。硫氧还蛋白参与蛋白质在发芽时的加工，已经报道了对谷物消化率的影响，并且在基因型间差异表达。因此，氧化还原状态的胚乳蛋白质，其中kafirins是一个子集，可以影响品质性状，除了蛋白质的表达。
Grain protein composition determines quality traits, such as value for food, feedstock, and biomaterials uses. The major storage proteins in sorghum are the prolamins, known as kafirins. Located primarily on the periphery of the protein bodies surrounding starch, cysteine-rich beta- and gamma- kafirins may limit enzymatic access to internally positioned alpha-kafirins and starch. An integrated approach was used to characterize sorghum with allelic variation at the kafirin loci to determine the effects of this genetic diversity on protein expression. Reversed-phase high performance liquid chromatography and lab-on-a-chip analysis. showed reductions in alcohol-soluble protein in beta-kafirin null lines. Gel-based separation and liquid chromatography-tandem. mass spectrometry identified a range of redox active proteins affecting storage protein biochemistry. Thioredoxin, involved in the processing of proteins at germination, has reported impact on grain digestibility and was differentially expressed across genotypes. Thus, redox states of endosperm proteins, of which kafirins are a subset, could affect quality traits in addition to the expression of proteins.