Automatic measurement of the red cell oxygen dissociation curve identical with the whole blood curve.
Automatic measurement of the red cell oxygen dissociation curve identical with the whole blood curve.
复制标题
自动测量红细胞氧解离曲线与全血曲线一致。
DOI：
10.2170/jjphysiol.49.379
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
K. Imai
中科院分区：
文献类型：
--
作者：
A. Mawjood;K. Imai
自动测量血液和血红蛋白的整个氧解离曲线（ODC）为评估其气体传输功能提供了有用的手段。 Imai等人先前开发的自动充氧装置。 (1970, 1981)，使用极谱法测定氧分压和分光光度法测定血红蛋白的氧饱和度，主要用于血红蛋白溶液的准确 ODC 测量。然而，它不适合红细胞悬浮液，因为由于红细胞的光散射，吸光度信号上叠加了显着的噪声。在本研究中，我们通过在光度系统中使用积分球克服了这个问题。通过广泛的测试，我们找到了获得红细胞氧合数据的最佳实验条件，这些数据在 ODC 的位置（氧亲和力）和形状（S 形特征）及其 pH 依赖性（玻尔效应）方面与全血数据相同。其精度比“Hemox-Analyzer”（技术咨询服务）、“Hem-O-Scan”（Aminco）等市售自动装置高。因此，我们的方法提供了一种简单方便的方法，用于从一滴全血中获得模拟全血 ODC 的准确 ODC。提出了我们的方法在血液储存对 ODC 的影响方面的应用，证明了我们的方法的有用性。
Automatic measurement of the entire oxygen dissociation curve (ODC) of blood and hemoglobin provides a useful means for evaluating their gas-transport function. The automatic oxygenation apparatus previously developed by Imai et al. (1970, 1981), which uses a polarographic determination of partial pressure of oxygen and a spectrophotometric determination of oxygen saturation of hemoglobin, has mostly been used for the measurement of accurate ODCs of hemoglobin solution. However, it was not suitable for red cell suspension because a significant noise was superimposed on the absorbance signal due to light-scattering by red cells. In the present study, we have overcome this problem by using an integrating sphere for the photometric system. Through extensive tests we found the optimal experimental conditions for obtaining the red cell oxygenation data that were identical with the whole blood data with respect to the position (oxygen affinity) and shape (sigmoid character) of the ODC and its pH-dependence (the Bohr effect). The accuracy was higher than that of commercially available automatic apparatuses such as the "Hemox-Analyzer" (Technical Consulting Service) and "Hem-O-Scan" (Aminco). Thus, our method provides an easy and convenient means for obtaining accurate ODCs mimicking the whole blood ODCs from one drop of whole blood. An application of our method to the effect of blood storage on ODC is presented, demonstrating the usefulness of our method.