A comparison of three methods for isolating RNA from mosquitoes.
A comparison of three methods for isolating RNA from mosquitoes.
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DOI：
10.1111/j.1365-2583.1993.tb00121.x
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发表时间：
1993-01-01
影响因子：
2.6
通讯作者：
Wells, M.A.
中科院分区：
文献类型：
--
作者：
Noriega, F.G.;Wells, M.A.
将两种最常见的 RNA 分离方案（Chirgwinet al., 1979 和 Chomczynski & Sacchi, 1987）与使用玻璃粉的方案进行了比较。使用琼脂糖凝胶和 Northern 杂交测试回收的 RNA 的质量。使用玻璃粉方案，我们始终获得完整的 RNA，而使用其他方案，我们经常获得降解的 RNA。使用特定的放射性探针，我们测量了从喂养的成年埃及伊蚊中回收的核糖体 RNA 和胰蛋白酶 mRNA。与 Chirgwin 和 Chomczynski 方案相比，我们的方案的恢复率分别提高了 40 倍和 3 倍。使用玻璃粉的方法简单、快速、重现性好，且完整 RNA 产量高。
The two most common protocols for RNA isolation, Chirgwinet al., 1979, and Chomczynski & Sacchi, 1987, were compared with a protocol using glass powder. The quality of the recovered RNA was tested using agarose gels and Northern hybridization. With the glass powder protocol, we consistently obtained intact RNA, whereas with the other protocols we often obtained degraded RNA. Using specific radioactive probes, we measured ribosomal RNA and trypsin mRNA recovered from fed adultAedes aegyptifemales. Our protocol gave a 40‐ and a 3‐fold increase in recovery when compared with the Chirgwin and Chomczynski protocol, respectively. The method using glass powder is simple, fast, reproducible and gives a high yield of intact RNA.