Distribution patterns of β- and γ-herpesviruses within Waldeyer's ring organs
Distribution patterns of β- and γ-herpesviruses within Waldeyer's ring organs
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DOI：
10.1002/jmv.20899
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发表时间：
2007-08-01
影响因子：
12.7
通讯作者：
Nadal, David
中科院分区：
文献类型：
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作者：
Berger, Christoph;Hug, Martina;Nadal, David
瓦尔德耶氏环是指咽内淋巴组织的一个功能单位，包括腺样体和扁桃体。为了深入了解(β -和γ -人疱疹病毒（hhv）的分布模式及其在自然入口的潜在相互影响，对30名儿童的腺样体和扁桃体进行了定量聚合酶链反应（qPCR）检测。在24例（80%）、19例（63%）、23例（77%）、23例（77%）和0例（0%）儿童的腺样体、扁桃体或两者中分别检测到eb病毒（EBV）、巨细胞病毒（CMV）、HHV-6、HHV-7和HHV-8的DNA。EBV、CMV、HHV-6和-7分别定位于92%、37%、52%和70%的儿童的腺样体和扁桃体，qPCR可检测到病毒。EBV的数量是其他hhv的2-10倍，且与自体器官相关（P=0.01）， HHV-7的数量也是如此（P=0.002）。CMV的数量与腺样体中HHV-6的数量（P= 0.028）、扁桃体中HHV-7的数量（P= 0.007）、腺样体中HHV-7的数量相关（P < 0.01）。在CMV DNA可检测的腺样体中，HHV-6 DNA水平低于未检测CMV DNA的腺样体（P=0.0062）。相反，与未检测到EBV DNA的腺样体相比，携带EBV DNA的腺样体的CMV和HHV-7水平更高（P=0.019和P=0.039）。因此，β -和γ - hhv在瓦尔德耶氏环器官中表现出不同的分布行为，并且似乎相互作用。这可能对免疫功能低下的宿主具有重要的医学意义，这些宿主可能会重新激活hhv，导致严重的发病率和死亡。
The Waldeyer's ring designates a functional unit of lymphoid tissue within the pharynx including the adenoids and tonsils. To gain insight into distribution patterns of (beta- and gamma-human herpesviruses (HHVs) and their potential mutual influences at their natural portal of entry, quantitative polymerase chain reaction (qPCR) assays were applied to adenoids and tonsils obtained from 30 children. DNA of Epstein-Barr virus (EBV), cytomegalovirus (CMV), HHV-6, HHV-7, and HHV-8 was detected in adenoids, tonsils, or both of 24 (80%), 19 (63%), 23 (77%), 23 (77%), and 0 (0%) children, respectively. EBV, CMV, HHV-6, and -7 localized in both adenoids and tonsils from 92%, 37%, 52%, and 70% of children, respectively, with the virus detectable by qPCR. The amount of EBV was 2-10-fold higher than of other HHVs and correlated in autologous organs (P=0.01) as did the amount of HHV-7 (P=0.002). The amount of CMV correlated with the HHV-6 amount in adenoids (P= 0.028) and tonsils (P= 0.007), and with the amount of HHV-7 in adenoids (P < 0.01). Levels of HHV-6 DNA were lower in adenoids with detectable CMV DNA than in adenoids without detectable CMV DNA (P=0.0062). Inversely, CMV and HHV-7 levels were higher in adenoids with than in adenoids without detectable EBV DNA (P=0.019 and P=0.039, respectively).Thus, beta- and gamma-HHV exhibit distinct distribution behaviors in Waldeyer's ring organs and seem to interact. This may be of medical importance in immunocompromised hosts who are likely to reactivate HHVs causing severe morbidity and death.