The role of the Sid1p kinase and Cdc14p in regulating the onset of cytokinesis in fission yeast
The role of the Sid1p kinase and Cdc14p in regulating the onset of cytokinesis in fission yeast
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DOI：
10.1093/emboj/19.8.1803
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发表时间：
2000-04-17
期刊：
影响因子：
11.4
通讯作者：
McCollum, D
中科院分区：
文献类型：
--
作者：
Guertin, DA;Chang, L;McCollum, D
有丝分裂和胞质分裂的协调对于确保适当的染色体分离和基因组稳定至关重要。在裂殖酵母中，sid基因(cdc7、cdc11、cdc14、spg1、sid1、sid2和sid4)定义了调节隔膜和胞质分裂的信号通路。在这里，我们描述了一种新的蛋白激酶Sid1p的特征，Sid1p在后期不对称地定位于一个纺锤体极体(SPB)。Sid1p的定位在内环收缩和隔膜合成期间保持不变，并在细胞分离之前消失。此外，我们还发现Cdc14p与Sid1p处于一个复合体中。上位性分析将Sid1p-CDc14p放在Spg1p-CDc7p的下游但在Sid2p的上游，最后，我们表明有丝分裂过程中细胞周期蛋白的分解不受SID途径失活的影响；事实上，CDc2-Cyclin活性的丧失促进了Sid1p-CDc14p与SPB的结合，可能提供了一种耦合细胞质分裂和有丝分裂退出的机制。
Coordination of mitosis and cytokinesis is crucial for ensuring proper chromosome segregation and genomic stability. In Schizosaccharomyces pombe, the sid genes (cdc7, cdc11, cdc14, spg1, sid1, sid2 and sid4) define a signaling pathway that regulates septation and cytokinesis. Here we describe the characterization of a novel protein kinase, Sid1p, Sid1p localizes asymmetrically to one spindle pole body (SPB) in anaphase. Sid1p localization is maintained during medial ring constriction and septum synthesis and disappears prior to cell separation. Additionally, we found that Cdc14p is in a complex with Sid1p. Epistasis analysis places Sid1p-Cdc14p downstream of Spg1p-Cdc7p but upstream of Sid2p, Finally, we show that cyclin proteolysis during mitosis is unaffected by inactivating the sid pathway; in fact, loss of Cdc2-cyclin activity promotes Sid1p-Cdc14p association with the SPB, possibly providing a mechanism that couples cytokinesis with mitotic exit.