miR-342-3p promotes osteogenic differentiation via targeting ATF3
miR-342-3p promotes osteogenic differentiation via targeting ATF3
复制标题
miR-342-3p 通过靶向 ATF3 促进成骨分化。
DOI：
10.1002/1873-3468.13282
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发表时间：
2018-12-01
期刊：
影响因子：
3.5
通讯作者：
Hong, Wei
中科院分区：
文献类型：
--
作者：
Han, Yawei;Zhang, Kun;Hong, Wei
microRNA（miRNAs）通过其多靶点参与了许多生理和病理过程。在这项研究中，发现miR-342- 3 p随着韧带骨化或骨质疏松而失调。我们证明沉默miR-342- 3 p会损害成骨细胞活性和基质矿化，而过表达miR-342- 3 p会显著促进成骨细胞分化。此外，miR-342- 3 p直接靶向转录激活因子3（ATF 3），其抑制前成骨分化相关基因的转录。此外，在未分化的前成骨细胞中，Enah/Vasp样（EVL）基因座的CpG岛存在较高的甲基化频率;然而，EVL CpG岛的去甲基化诱导成骨分化过程中miR-342- 3 p的过度表达。提示miR-342- 3 p可能作为诊断和治疗韧带骨化和骨质疏松的潜在标志物。
Through their multiple targets, microRNAs (miRNAs) are involved in numerous physiological and pathological processes. In this study, miR-342-3p was found to be deregulated with ossification of ligament or osteoporosis. We demonstrate that silencing miR-342-3p impairs osteoblast activity and matrix mineralization, while over expression of miR-342-3p promotes osteoblast differentiation significantly. Moreover, miR-342-3p directly targets activating transcription factor 3 (ATF3), which inhibits transcription of pro-osteogenic differentiation-associated genes. In addition, there exists a higher frequency of methylation at the CpG island of the Enah/Vasp-Like (EVL) locus in undifferentiated pre-osteoblasts; however, demethylation of the EVL CpG island induces over expression of miR-342-3p during osteogenic differentiation. This study suggests that miR-342-3p may serves as a potential marker for diagnosis and treatment of ossification of ligament and osteoporosis.