Behavioral and pharmacological modulation of ventral tegmental dendritic dopamine release.
Behavioral and pharmacological modulation of ventral tegmental dendritic dopamine release.
复制标题
腹侧被盖树突多巴胺释放的行为和药理学调节。
DOI：
10.1016/0006-8993(94)91366-8
复制
发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Stein,EA
中科院分区：
文献类型：
--
作者：
Zhang,H;Kiyatkin,EA;Stein,EA
中皮质边缘多巴胺（DA）系统被认为是参与行为强化的神经基质的一部分。虽然已经把重点放在了mesolimbic终端领域的活动，体树突DA释放的存在也已成立。在本研究中，释放内源性DA从腹侧被盖DA细胞体区域的自由活动的大鼠进行了测量，使用在体内计时电流后，介绍了几种环境刺激。虽然介绍了9-s中等强度的光刺激没有显着修改DA释放，可口的食物介绍（FD），3分钟的社会互动（SI）与另一只雄性大鼠和3分钟的尾巴捏（TP）产生的电化学信号的增加，表明增加体树突DA释放。FD、SI和TP刺激后，平均峰值信号增加（以nM DA浓度校准）分别为104、135和161 nM。在每天呈现所有四种刺激后，给予四种DA活性药物之一s.c.与注射前相比，阿扑吗啡（APO）引起的电化学信号降低，而诺米芬辛（NOMI），可卡因（COC）和氟哌啶醇（HALO）引起的电化学信号增加。当TP给药后30分钟，APO，NOMI和COC抑制TP诱导的信号增加相比，药物注射前的反应。另一方面，HALO并不改变TP诱导的DA释放。这些数据支持的假设，即起源于mesocorticolimbic系统的细胞在生理相关的刺激后释放树突状DA。
The mesocorticolimbic dopamine (DA) system is thought to comprise part of a neural substrate participating in behavioral reinforcement. While much emphasis has been placed on mesolimbic terminal field activity, the existence of somatodendritic DA release has also been established. In the present study, the release of endogenous DA from the ventral tegmental DA cell body region of freely moving rats was measured, using in vivo chronoamperometry after presentation of several environmental stimuli. While presentation of a 9-s moderately intense light stimulus did not significantly modify DA release, palatable food presentation (FD), 3 min of social interaction (SI) with another male rat and a 3-min tail-pinch (TP) produced an increase in electrochemical signal, suggesting an increase in somatodendritic DA release. Mean peak signal increases (calibrated in nM DA concentration) were 104, 135 and 161 nM after FD, SI and TP stimulus, respectively. After daily presentation of all four stimuli, one of four DA active drugs was given s.c. When compared with preinjection baseline, apomorphine (APO) caused a decrease while nomifensine (NOMI), cocaine (COC) and haloperidol (HALO) caused an increase in electrochemical signal. When TP was given 30 min after drug injection, APO, NOMI and COC suppressed the TP-induced signal increase compared with the predrug response. HALO, on the other hand, did not alter TP-induced DA release. These data support the hypothesis that the cells of origin of the mesocorticolimbic system release dendritic DA after physiologically relevant stimuli.