Transcriptional regulation of the Drosophila-raf proto-oncogene by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system.
Transcriptional regulation of the Drosophila-raf proto-oncogene by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system.
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DOI：
10.1093/nar/25.4.794
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发表时间：
1997-02
影响因子：
14.9
通讯作者：
Jae-Ryeon Ryu;T. Choi;Eun-Jeong Kwon;Wonho Lee;Yasuyoshi Nishida;Y. Hayashi;A. Matsukage;Masamitsu Yamaguchi;Mi-Ae Yoo
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文献类型：
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作者：
Jae-Ryeon Ryu;T. Choi;Eun-Jeong Kwon;Wonho Lee;Yasuyoshi Nishida;Y. Hayashi;A. Matsukage;Masamitsu Yamaguchi;Mi-Ae Yoo
DRE/DREF系统在DNA复制基因的转录中起重要作用，例如编码DNA聚合酶α的180和73 kDa亚基以及编码PCNA的基因。在本研究中，我们在D-raf基因的5 '侧翼区（相对于转录起始位点的-370至-357）发现了两个与DRE同源的序列（5'-TATCGATA-3 '），并通过凝胶迁移率变化分析、瞬时CAT分析和携带D-raf和lacZ融合基因的转基因幼虫组织中lacZ表达的空间模式证实了D-raf基因的转录活性。此外，我们证明了D-raf基因是Zen蛋白的另一个靶点，并观察到Zen蛋白在培养细胞中的D-raf抑制及其在纯合Zen突变胚胎背侧区域的异位表达。DRE/DREF参与D-raf基因调控的证据有力地支持了DRE/DREF系统负责果蝇细胞增殖相关基因的协调调控的观点。
The DRE/DREF system plays an important role in transcription of DNA replication genes such as those encoding the 180 and 73 kDa subunits of DNA polymerase alpha as well as that for encoding PCNA. In this study, we found two sequences homologous to DRE (5'-TATCGATA-3') in the 5'-flanking region (-370 to -357 with respect to the transcription initiation site) of the D-raf gene and confirmed transcriptional activity through gel mobility shift assays, transient CAT assays, and spatial patterns of lacZ expression in transgenic larval tissues carrying D-raf and lacZ fusion genes. Further, we demonstrated that the D-raf gene is another target of the Zerknüllt (Zen) protein with observation of D-raf repression by Zen protein in cultured cells and its ectopic expression in the dorsal region of the homozygous zen mutant embryo. The evidence of DRE/DREF involvement in regulation of the D-raf gene obtained in this study strongly supports the idea that the DRE/DREF system is responsible for the coordinated regulation of cell proliferation-related genes in Drosophila.